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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (24): 187-192.doi: 10.11924/j.issn.1000-6850.2013-0653

所属专题： 油料作物； 园艺

• 植物保护 农药 • 上一篇    下一篇

陕西关中地区甘蓝型油菜白粉病的病原菌鉴定

郭徐鹏 李建昌 李永红 田建华 赵小光 张耀文 王灏 李殿荣   

  • 收稿日期:2013-03-11 修回日期:2013-04-05 出版日期:2013-08-25 发布日期:2013-08-25
  • 基金资助:
    油菜现代产业技术体系建设

The Identification of the Pathogen Causing Rape Powdery Mildew in Guanzhong Area of Shaanxi Province

  • Received:2013-03-11 Revised:2013-04-05 Online:2013-08-25 Published:2013-08-25

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摘要： 为明确陕西关中地区甘蓝型油菜白粉病的病原种类，通过显微观察以及分子鉴定方法，对引起陕西关中地区油菜白粉病的病原进行鉴定。病原菌分生孢子梗简单不分枝，分生孢子顶生，无色，椭圆形或桶柱形，未发现闭囊壳。采用rDNA ITS通用引物扩增了病菌ITS区，对其产物进行测序及系统发育分析，5株菌与Erysiphe cruciferarum聚在一个独立的分枝上，支持率为96%。结合形态学和系统发育分析，鉴定陕西关中地区甘蓝型油菜白粉菌病原为Erysiphe cruciferarum。

关键词: 相关分析, 相关分析

Abstract: The pathogen causing powdery mildew on Brassica napus L. in Shaanxi province, was indentificated by morphological characterisation and phylogenetic analysis. Conidiophores of pathogen were unbranched. conidia were brone singly, hyphae, cylindrical, or ellipsoid-cylindrica. No chasmothecia were found. The ITS region of rDNA was amplified with universal primers and sequenced. The collected five strains and the two Erysiphe cruciferarum strains fell within a single clade with 96% bootstrap support by established the parsimony tree. Based on phylogenetic analysis and morphological characters of the anamorph, we identi?ed the collected five strains as Erysiphe cruciferarum in Shaanxi province.