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中国农学通报 ›› 2013, Vol. 29 ›› Issue (34): 66-70.doi: 10.11924/j.issn.1000-6850.2013-1042

所属专题: 生物技术 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

不同烤烟品种碳氮及其次生代谢相关酶的动态变化研究

陈波 潘文杰 陈伟 孟玉山 宗学凤 王三根   

  • 收稿日期:2013-04-11 修回日期:2013-05-28 出版日期:2013-12-05 发布日期:2013-12-05

The Dynamic Changes on Secondary Enzymes Activeness of Different Flue-cured Tobacco Leaves in Carbon and Nitrogen Metabolism

  • Received:2013-04-11 Revised:2013-05-28 Online:2013-12-05 Published:2013-12-05

摘要: 为烤烟品种的推广应用及其品质鉴定提供理论支撑,以‘云烟87’、‘遵烟6号’和‘K326’为材料,研究了不同品种烤烟在移栽后0—45天碳氮及其次生代谢相关酶活性的动态变化。结果表明,(1)3种烤烟品种淀粉酶都随移栽时间呈逐渐上升的趋势,其中移栽后45天‘云烟87’的淀粉酶活性值最大。(2)‘云烟87’烟叶蔗糖合成酶活性随着生育期迅速上升;‘遵烟6号’烟叶蔗糖合成酶活性生育期相对稳定。‘K326’烟叶蔗糖合成酶活性呈现先下降后上升的趋势,与其他品种相比,在移栽后30—45天酶活性较低。(3)‘云烟87’的烟叶蔗糖转化酶活性先缓慢上升,30天后较为稳定;‘遵烟6号’烟叶蔗糖转化酶活性在生育期一直较为稳定;‘K326’的烟叶蔗糖转化酶活性呈现出先升后降的变化趋势。移栽后30—45天,‘云烟87’和‘遵烟6号’烟叶蔗糖转化酶活性都高于‘K326’。(4)‘云烟87’硝酸还原酶活性在上升至15天后较稳定,‘遵烟6号’表现出较大的波动,‘K326’呈现逐渐下降的趋势。(5)‘云烟87’烟叶的苯丙氨酸裂解酶活性呈现先略微升高随后下降的变化趋势;‘遵烟6号’和‘K326’均随着生育期呈现升-降-升的变化趋势。总之,‘云烟87’具有更强的碳氮代谢和次生物质代谢能力。

关键词: 木质素, 木质素

Abstract: Using Yunyan87,Zunyan6 and k326 as materials, the dynamic changes on secondary enzymes activeness of the three flue-cured tobacco leaves in carbon and nitrogen metabolism were studied. The result showed that, (1)The activity of amylase in all three flue-cured tobacco leaves rose as transplant time went by. After 30th day, the increasing speed of all flue-cured tobacco was faster than before. The activity of amylase in Yunyan87 was highest between 15 and 45 day after transplanting. (2)The Sucrose synthase activity in Zunyan6 was relatively stable in 45 days, in which Yunyan87cut down quickly, at30th day, the lowest, then kept. on the contrary, the Sucrose synthase activity in Yunyan87 went up rapidly. Between 30th and 45th day, which in k326 was lowest. (3)The Invertase activity in Yunyan87 went up slowly at the beginning, and then changing less. In k326, it increased and then decreased, at 15th day, the maximum. The Invertase activity in Zunyan6 did not change much. Between 30 and 45 day after transplanting, Zunyan6 was lower than the other two. (4)The nitrate reductase activity of the three flue-cured tobacco leaves was significantly different, Yunyan87 rose and then stable, but Zunyan6 changed a lot, while k326 went down continuously. At the 15th day, the nitrate reductase activity in Zunyan6 was highest, Yunyan87 and k326 t had no significant difference. At 45th day, Zunyan6 was higher than other, and k326 was lowest. (5)The Phenylalanine lyase activity in Yunyan87 took on an slowly increase and then decreased trend; Zunyan6 and k326 changed in the same trend, up-down-up, but Zunyan6 changed much more than k326. It showed that Yunyan87 had more abilities in secondary enzymes.