关键词: 蓝莓, 蓝莓, 正交试验, 生长, pH, 控水, 施肥

Abstract: The aim is to provide a basis for verifying the protein function of coumarate 3-hydroxylase gene (C3H) in the later period, and improving the genetic engineering breeding of Boehmeria nivea variety with low content lignin. Based on the information of high-throughput sequencing of Boehmeria nivea, the full-length sequence of C3H was cloned by the RACE technology, and then the bioinformatics analysis was conducted. The expression pattern of C3H was tested respectively by fluorescent quantitative technology.Bn C3H-1 (cDNA sequence of C3H) with 1940 bp in length was obtained (GenBank accession number: KY078743). The analysis of the physical and chemical properties and structure of BnC3H-1 gene encoding protein showed that the gene encoded a hydrophilic protein containing 511 amino acids with a molecular weight of 57.80 kD, it might be localized in the endoplasmic reticulum. According to the sequence of amino acid and structural analysis, BnC3H-1 contained one conserved domain, cytochrome P450 domain. Phylogenetic analysis showed that the gene had high homology with C3H genes of Eucalyptus grandis and Fagopyrum tataricum.