中国农学通报 ›› 2014, Vol. 30 ›› Issue (6): 1-12.doi: 10.11924/j.issn.1000-6850.2013-1123
• 农学 农业基础科学 • 下一篇
何秀英 王玲 吴伟怀 陈钊明
收稿日期:
2013-04-17
修回日期:
2013-06-17
出版日期:
2014-02-25
发布日期:
2014-02-25
基金资助:
Received:
2013-04-17
Revised:
2013-06-17
Online:
2014-02-25
Published:
2014-02-25
摘要: 稻瘟病是一种世界性的水稻病害,抗病品种的培育和种植是控制该病害最为经济有效的方法,而抗病基因的发掘与利用则是抗病育种的基础和核心。本研究就稻瘟病抗病基因的遗传、定位、克隆及育种应用情况进行了概述,介绍了稻瘟病广谱抗原和抗病基因、隐性抗病基因研究的最新进展,指出近一半的抗病基因是通过F2分离群体鉴定的,目前已定位的稻瘟病主效抗病基因超过86个,微效基因约350个,应用图位克隆等方法,20个稻瘟病主效抗病基因和2个微效基因已从不同的水稻品种中被克隆。这些基因的定位和克隆是有效开展稻瘟病抗性分子育种的基础。最后,结合笔者从事水稻稻瘟病抗性遗传的工作实践对稻瘟病抗病基因研究存在的问题进行了分析和展望,相信随着越来越多各类型抗性基因的生产应用,稻瘟病对水稻的危害最终能得到有效的控制。
何秀英 王玲 吴伟怀 陈钊明. 水稻稻瘟病抗性基因的定位、克隆及育种应用研究进展[J]. 中国农学通报, 2014, 30(6): 1-12.
[1] Dean R A, Talbot N J, Ebbole D J, et al. The genome sequence of the rice blastfungus Magnaporthe grisea[J]. Nature,2005,434: 980-986. [2] Baker B, Zambryski P, Staskawicz B, et al. Signaling in plant-microbe interations[J]. Science,1997,276:726-733. [3] Moytri R,贾育林,Richard D C.水稻抗稻瘟病基因的结构、功能和共同进化[J].作物学报,2012,38(3):381-393. [4] 孙国昌,杜新法,陶荣祥,等.水稻稻瘟病防治研究进展和 21世纪初研究设想[J].植物保护学报,2000,26(1):33-35. [5] 全国农业技术推广中心.2012年全国农作物重大病虫害呈重发态势[J].农药市场信息,2012(5):49. [6] 江南,刘雄伦,戴良英,等.水稻抗稻瘟病基因的定位与克隆研究进展[J].中国农学通报,2010,26(10):270-275. [7] Zhang Y X, Yang J Y, Shan Z L, et al. Substitution mapping of QTLs for blast resistance with SSSLs in rice (Oryza sativa L.) [J]. Euphytica,2012,184(1):141-150. [8] 雷财林,凌忠专,王久林,等.水稻抗病育种研究进展[J].生物学通报, 2004,39(11):4-7. [9] 何秀英,廖耀平,陈钊明,等.水稻稻瘟病抗病育种研究进展与展望[J].广东农业科学,2011,(1):30-33. [10] Sasaki R. Existence of strains in rice blast fungus[J]. Japanese Journal of Plant Protection,1922,9:631-644. [11] 凌忠专.稻瘟病研究论文集 [M].北京:中国农业出版社,2005: 231-242. [12] 山崎義人,高坂淖尔编著.稻瘟病与抗病育种[M]. 凌忠专,孙昌其译,林世成校.北京:农业出版社,1990:180-224. [13] 吴俊,刘雄伦,戴良英,等.水稻广谱抗稻瘟病基因研究进展[J].生命科学,2007,19(2):233-237. [14] Ballini E, Morel J, Droc G, et al. A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance[J]. Molecular Plant-Microbe Interactions,2008,21(7):859-868. [15] Liu Y, Liu B, Zhu X, et al. Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice[J]. Theoretical and Applied Genetics, DOI 10.1007// s00122-012-2031-3, published online:12 February 2013. [16] 杨勤忠,林菲,冯淑杰,等.水稻稻瘟病抗性基因的分子定位及克隆研究进展[J].中国农业科学,2009,42(5):1601-1615. [17] Sharma T R, Rai A K, Gupta S K, et al. Rice blast management through host-plant resistance: retrospect and prospects[J]. Agricultural Research,2012,1(1):37-52. [18] Chen S, Wang L, Que Z, et al. Genetic and physical mapping of Pi37(t), a new gene conferring resistance to rice blast in the famous cultivar St. No. 1[J]. Theoretical and Applied Genetics,2005,111(8): 1563-1570. [19] Kaji R, Ogawa T, Nishimura M. RFLP mapping of a blast resistance gene, Pit, in rice[J]. Breeding Science (Suppl. 1),1997,47:37. [20] Araki E, Yanoria M J T, Ebron L A, et al. Mapping of a rice blast resistance gene Pish[J]. Breeding Research(Suppl. 2),2003,5:333. [21] Takahashi A, Hayashi N, Miyao A, et al. Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging[J]. BMC Plant Biology,2010,10:175. [22] Zhu M, Wang L, Pan Q. Identification and Characterization of a new blast resistance gene located on rice chromosome 1 through linkage and differential analyses[J]. Phytopathology,2004,94(5): 515-519. [23] Nguyen T T T , Koizumi S, La T N, et al. Pi35(t), a new gene conferring partial resistance to leaf blast in the rice cultivar Hokkai 188[J]. Theoretical and Applied Genetics,2006,113(4):697-704. [24] Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes[J]. The Plant Journal, 1999,19(1):55-64. [25] Pan Q H, Wang L, Ikehashi H, et al. Identification of two new genes conferring resistance to rice blast in the Chinesenative cultivar‘Maowangu’ [J]. Plant Breeding,1998,117(1):27-31. [26] Pan Q H, Wang L, Tanisaka T. A new blast resistance gene identified in the Indian native rice cultivar Aus373 through allelism and linkage tests[J]. Plant Pathology,1999,48(2):288-293. [27] Chen X W, Li S G, Xu J C, et al. Identification of Two Blast Resistance Genes in a Rice Variety, Digu[J]. Journal of Phytopathology, 2004,152(2):77-85. [28] Zhou J H, Wang J L, Xu J C, et al. Identification and mapping of a rice blast resistance gene Pi-g(t) in the cultivar Guangchangzhan[J]. Plant Pathology, 2004,53(2):191-196. [29] Tabien R E, Li Z, Paterson A H, et al. Mapping of four major rice blast resistance genes from‘Lemont’ and ‘Teqing’ and evaluation of their combinatorial effect for field resistance[J]. Theor Appl Genet, 2000,101(8):1215-1225. [30] Goto I. Genetic studies on resistance of rice plant to blast fungus ( Ⅶ). Blast resistance genes of Kuroka[J]. Annals of Phytopathological Society of Japan,1988,54:460-465. [31] Monosi B, Wisser R J, Pennill L, et al. Full-genome analysis of resistance gene homologues in rice[J]. Theoretical Applied Genetics,2004,109(7):1434-1447. [32] Fukuoka S, Okuno K. QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice[J]. Theoretical and Applied Genetics,2001,103:185-190. [33] Naqvi N I, Chattoo B B. Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice[J]. Genome,1996,39(1): 26-30. [34] Ahn S N, Kim Y K, Hong H C, et al. Molecular mapping of genes for resistance to Korean isolates of rice blast. In harmonizing agricultural productivity and conservation of biodiversity breeding and ecology. Proceed. 8th SABRAO Congress and Annual Meeting of Korean Breeding Socienty,1997:435-436. [35] Inukai T, Mackill D J, Bonman J M, et al. Blast resistance genes Pi-2(t) and Pi-z may be allelic[J]. Rice Genetics Newsletters,1992,9: 90-92. [36] Hayashi K, Yoshida H, Ashikawa I. Development of PCR-based allele-specific and InDel markers sets for nine rice blast resistance genes. Theoretical and Applied Genetics,2006,113:251-260. [37] Liu G, Lu G, Zeng L, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6 [J]. Molecular Genetics and Genomics,2002,267(4):472-480. [38] Yokoo M, Kiyosawa S. Inheritance of blast resistance of the rice variety, Toride 1, selected from the cross Norin 8 × TKM.1[J]. Japanese Journal of Breeding,1970,20(3):129-132. [39] Zhou B, Qu S, Liu G, et al. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions,2006,19(11):1216-1228. [40] Deng Y, Zhu X, Shen Y, et al. Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety[J]. Theoretical and Applied Genetics,2006,113:705-713. [41] Zhu X Y, Chen S, Yang J Y, et al. The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family [J]. Theoretical and Applied Genetics,2012,124:1295-1304. [42] Shang J, Tao Y, Chen X, et al. Identification of a new rice blast resistance gene, Pid3, by genomewide comparison of paired Nucleotide-Binding Site-Leucine-Rich Repeat genes and their pseudogene alleles between the two sequenced rice genomes[J]. Genetics,2009,182(4):1303-1311. [43] Wu J L, Fan Y Y, Li D B, et al. Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates[J]. Theoretical and Applied Genetics,2005,111(1):50-56. [44] Chen J, Shi Y, Liu W, et al. A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae[J]. Journal of Genetics and Genomics,2011,38(5):209-216. [45] Pan Q, Wang L, Ikehashi H, et al. Identification of a new blast resistance gene in the indica rice cultivar Kasalath using Japanese differential cultivars and isozyme markers[J]. Phytopathology, 1996,86(10):1071-1075. [46] Jeung J U, Kim B R, Cho Y C, et al. A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice[J]. Theoretical and Applied Genetics,2007,115(8):1163-1177. [47] Pan Q H, Tanisaka T, Ikehashi H. Studies on the genetics and breeding of blast resistance in rice VI. Gene analysis for the blast resistance of two Yunnan native cultivars GA20 and GA25[J]. Breeding Science,1996,46(Suppl.2):70. [48] 朱立煌,徐吉臣,陈英,等.用分子标记定位一个未知的抗稻瘟病基因[J].中国科学(B辑),1994,24(10):1048-1052. [49] Berruyer R, Adreit H, Milazzo J, et al. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1[J]. Theoretical and Applied Genetics,2003,107(6):1139-1147. [50] Liu X Q, Wang L, Chen S, et al. Genetic and physical mapping of Pi36(t), a novel rice blast resistance gene located on rice chromosome 8[J]. Molecular Genetics and Genomics,2005,274(4): 394-401. [51] Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two Coiled-CoilNucleotide-Binding-Leucine-Rich repeat genes[J]. Genetics,2009, 181(4):1627-1638. [52] He X Y, Liu X Q, Wang L, et al. Identification of the new recessive gene pi55(t) conferring resistance to Magnaporthe oryzae[J]. Science China Life Sciences,2012,55(2):141-149. [53] Liu B, Zhang S H, Zhu X, et al. Candidate defense genes as predictors of quantitative blast resistance in rice[J]. Molecular Plant-Microbe Interactions,2004,17(10):1146-1152. [54] Jeon J S, Chen D, Yi G H, et al. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast[J]. Molecular Genetics and Genomics,2003,269(2):280-289. [55] Lin F, Liu Y, Wang L, et al. A high-resolution map of the rice blast resistance gene Pi15 constructed by sequence-ready markers[J]. Plant Breeding,2007,126(3):287-290. [56] Inukai T, Nelson R J, Zeigler R S, et al. Allelism of blast resistance genes in near-isogenic lines of rice[J]. Phytopathology,1994,84(11), 1278-1283. [57] Zeng X, Yang X, Zhao Z, et al. Characterization and fine mapping of the rice blast resistance gene Pia[J]. Science China Life Sciences, 2011,54(4):372-378. [58] Shinoda H, Toriyama K, Yunoki T, et al. Studies on the varietal resistance of rice to blast, 6. Linkage relationship of blast resistance genes[J]. Bulletin of the Chugoku Agricultural Experiment Station, 1971,20:1-25. [59] 李培富,史晓亮,王建飞,等.太湖流域粳稻地方品种黑壳子粳抗稻瘟病基因的分子定位[J].中国水稻科学,2007,21(6):579-584. [60] Fjellstrom R, Conaway B C A, McClung A M, et al. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes [J]. Crop Science,2004,44(5):1790-1798. [61] Zhai C, Lin F, Dong Z, et al. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication[J]. New Phytologist,2011,189:321-334. [62] Yu Z H, Mackill D J, Bonman J M, et al. Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.) [J]. Theoretical and Applied Genetics,1996,93:859-863. [63] Xu X, Hayashi N, Wang C T, et al. Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates[J]. Molecular Breeding,2008,22(2): 289-299. [64] Li L Y, Wang L, Jing J X, et al. The Pikm gene, conferring stable resistance to isolates of Magnaporthe oryzae, was finely mapped in a crossover-cold region on rice chromosome 11[J]. Molecular Breeding,2007,20(2):179-188. [65] Wang L, Xu X, Lin F, et al. Characterization of Rice Blast Resistance Genes in the Pik Cluster and Fine Mapping of the Pik-p Locus[J]. Phytopathology,2009,99(8):900-905. [66] Pan Q H, Wang L, Tanisaka T, et al. Allelism of rice blast resistance genes in two Chinese rice cultivars, and identification of two new resistance genes[J]. Plant Pathology,1998,47(2):165-170. [67] Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics,1994,136:1421-1434. [68] Campbell M A, Chen D, Ronald P C. Development of co-dominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7(t) in recombinant inbred line 29[J]. Phytopathology,2004,94(3):302-307. [69] Ahn S N, Kim Y K, Hong H C, et al. Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.) [J]. Euphytica,2000,116(1):17-22. [70] Zenbayashi K, Ashizawa T, Tani T, et al. Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32[J]. Theoretical and Applied Genetics,2002, 104:547-552. [71] Gowda M, Roy-Barman S, Chattoo B. B. Molecular mapping of a novel blast resistance gene Pi38 in rice using SSLP and AFLP markers[J]. Plant Breeding,2006,125(6):596-599. [72] Chen D H, Vina M D, Inukai T, et al. Molecular mapping of the blast resistance gene, Pi44(t) in a line derived from a durably resistant rice cultivar[J]. Theoretical and Applied Genetics,1999,98: 1046-1053. [73] Fujii K, Hayano S Y, Saito K, et al. Identification of a RFLP marker tightly linked to the panicle blast resistance gene, Pb1, in rice[J]. Breeding Science,2000,50(3):183-188. [74] Chauhan R S, Farman M L, Zhang H B, et al. Genetic and physical mapping of a rice blast resistance locus, Pi-CO39(t), that corresponds to the avirulence gene AVR1-CO39 of Magnaporthe grisea[J]. Molecular Genetics and Genomics,2002,267(5):603-612. [75] 张建福,王国英,谢华安,等.粳稻云引抗稻瘟病基因的遗传分析及其定位[J].农业生物技术学报,2003,11(3):241-244. [76] 张锦文,谭亚玲,洪汝科,等.高原粳稻子预 44抗稻瘟病基因遗传分析和定位[J].中国水稻科学,2009,23(1):31-35. [77] Rybka K, Miyamoto M, Ando I, et al. High resolution mapping of the indica-derived rice resistance genes Ⅱ. Pita2 and Pita and a consideration of their origin[J]. Molecular Plant-Microbe Interactions,1997,10(4):517-524. [78] Hittalmani S, Parco A, Mew T V, et al. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J]. Theoretical and Applied Genetics,2000,100: 1121-1128. [79] Causse M A, Fulton T M, Cho Y G, et al. Saturated molecular map of the rice genome based on an interspecific backcross population [J]. Genetics,1994,138(4):1251-1274. [80] Hayashi N, Ando I, Imbe T. Identification of a new resistance gene to a Chinese blast fungus isolate in the Japanese rice cultivar Aichi Asahi[J]. Phytopathology,1998,88(8):822-827. [81] Imbe T, Oba S,Yanoria M J T, et al. A new gene for blast resistance in rice cultivar, IR24[J]. Rice Genetics Newsletters,1997,14:60-62. [82] Zhuang J Y, Ma W B, Wu J L, et al. Mapping of leaf and neck blast resistance genes with resistance gene analog, RAPD and RLP in rice [J]. Euphytica,2002,128(3):363-370. [83] Liu X Q, Yang Q Z, Lin F, et al. Identification and fine mapping of Pi39(t), a major gene conferring the broad-spectrum resistance to Magnaporthe oryzae[J]. Molecular Genetics and Genomics,2007, 278(4):403-410. [84] Yang Q Z, Lin F, Wang L, et al. Identification and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sativa subsp. indica reference cultivar, 93-11[J]. Theoretical and Applied Genetics,2009,118:1027-1034. [85] Kumar P, Pathania S, Katoch P, et al. Genetic and physical mapping of blast resistance gene Pi-42(t) on the short arm of rice chromosome 12[J]. Molecular Breeding,2010,25(2):217-228. [86] 郑康乐,钱惠荣,庄杰云,等.应用 DNA标记定位水稻的抗稻瘟病基因[J].植物病理学报,1995,25(4):307-313. [87] Naqvi N I, Chattoo B B. Molecular genetic analysis and sequence characterized amplified region-assisted selection of blast resistance in rice. In Khush G S. Rice Genetics Ⅲ. IRRI, Manila, The Philippines,1996:570-576. [88] Liu J L, Wang X J, Mitchell T, et al. Recent progress and understanding of the molecular mechanisms of the rice-Magnaporthe oryzae interaction[J]. Molecular Plant Pathology, 2010,11(3):419-427. [89] Das A, Soubam D, Singh P K, et al. A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae[J]. Functional & Integrative Genomics,2012,12:215-228. [90] Takahashi J S, Pinto L H, Vitaterna M H. Forward and reverse genetic approaches to behavior in the mouse[J]. Science,1994,264 (5166):1724-1733. [91] 刘鹏,魏毅东,陈由强,等.水稻稻瘟病抗性基因的归类分析及其功能研究进展[J].分子植物育种,2011,9(2):128-135. [92] 刘杨,肖应辉,史学涛,等.基因克隆策略在抗稻瘟病基因分离中的应用[J].中国农学通报,2012,28(24):166-172. [93] Coulson A, Sulston J, Brenner S, et al. Toward a physical map of the genome of the nemtode Caenorhabditis elegans[J]. Proceeding of the National Academy of Sciences of the United States of America,1986,83(20):7821-7825. [94] Yuan B, Zhai C, Wang W, et al. The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes[J]. Theoretical and Applied Genetics,2011,122 (5):1017-1028. [95] Lin F, Chen S, Que Z, et al. The blast resistance gene Pi37 encodes a Nucleotide Binding Site-Leucine-Rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1[J]. Genetics,2007,177(3):1871-1880. [96] Hayashi K, Yoshida H. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter[J]. The Plant Journal,2009,57(3):413-425. [97] Fukuoka S, Saka N, Koga H, et al. Loss of function of a praline-containing protein confers durable disease resistance in rice [J]. Science,2009,325(5943):998-1001. [98] Qu S, Liu G, Zhou B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site – leucine-rich repeat protein and is a member of a multigene family in rice[J]. Genetics, 2006,172:1901-1914. [99] Deng Y, Zhu X, Xu J, et al. Map-baesd cloning and breeding application of a broad-spectrum resistance gene Pigm to rice blast. Wang G L, Valent B(eds). Advances in Genetics, Genomics and Control of Rice Blast Disease(M),2009,PartⅡ,161-171. [100] Chen X , Shang J, Chen D, et al. A B-lectin receptor kinase gene conferring rice blast resistance[J]. The Plant Journal,2006,46(5): 794-804. [101] Liu X Q, Lin F, Wang L, et al. The in Silico Map-Based Cloning of Pi36, a rice Coiled-Coil-Nucleotide-Binding Site-Leucine-Rich Repeat gene that confers race-specific resistance to the blast fungus [J]. Genetics,2007,176(4):2541-2549. [102] Sharma T R, Madhav M S, Singh B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea[J]. Molecular Genetics and Genomics,2005,274(6):569-578. [103] Ashikawa I, Hayashi N, Yamane H, et al. Two Adjacent Nucleotide-Binding Site-Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance[J]. Genetics,2008,180(4):2267-2276. [104] Hua L, Wu J, Chen C, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast[J]. Theoretical and Applied Genetics,2012,125(5):1047-1055. [105] Okuyama Y, Kanzaki H, Abe A, et al. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes[J]. The Plant Journal,2011,66(3):467-479. [106] Hayashi N, Inoue H, Kato T, et al. Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication [J]. The Plant Journal,2010,64(3):498-510. [107] Bryan G T, Wu K S, Farrall L, et al. A Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta[J]. The Plant Cell,2000,12(11): 2033-2046. [108] Martin G B, Bogdanove A J, Sessa G. Understanding the functions of plant disease resistance proteins[J]. Annual Review of Plant Biology,2003,54:23-61. [109] Liu J L, Liu X L, Dai L Y, et al. Recent progress in elucidating the structure, function and evolution of disease resistance genes in plants[J]. Journal of Genetics Genomics,2007,34(9):765-776. [110] 沈瑛,Adreit H,朱旭东,等.中国部分杂交稻和常规早籼、晚粳品种(系)的抗瘟性[J].中国农业科学,2004,37(3):362-369. [111] 伍尚忠,朱小源,刘斌,等.籼稻品种三黄占 2号的稻瘟病持久抗性评价与遗传分析[J].中国农业科学,2004,37(4):528-534. [112] Huang H, Huang L, Feng G, et al. Molecular mapping of the new blast resistance genes Pi47and Pi48 in the durably resistant local rice cultivar Xiangzi 3150[J]. Phytopathology,2010,101(5):620-626. [113] Mackill D J, Bonman J M. Inheritance of blast resistance in near-isogenic lines of rice[J]. Phytopathology,1992,82(7):746-749. [114] Li W, Lei C L, Cheng Z J, et al. Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding[J]. Molecular Breedimg,2008,22:141-149. [115] Suh J P, Roh J H, Cho Y C, et al. The Pi40 gene for durable resistance to rice blast and molecular analysis of Pi40-advanced backcross breeding lines[J]. Phytopathology, 2009,99(3):243-250. [116] 翟文学.水稻隐性抗白叶枯病基因的克隆[J].四川农业科技,2006 (4):24. [117] 梁斌,余腾琼,徐福荣,等.云南省 3个地方稻种的抗稻瘟病性遗传分析[J].中国农业科学,2002,35(7):784-788. [118] 钟义明,江光怀,陈学伟,等.水稻含隐性抗白叶枯病基因 xa5的 24 kb片段的鉴定与基因预测[J].科学通报,2003,48(19):2057-2061. [119] 储昭晖.水稻白叶枯病隐性抗病基因 xa13 的分离与鉴定的研究[J].华中农业大学学报,2011,30(3):390-392. [120] 刘二明.水稻稻瘟病病圃建立及抗病育种优选评价[D].北京:中国农业科学院博士后研究报告,2004. [121] 邓其明,周鹏,林琳,等.水稻稻瘟病抗性基因研究进展及其在育种上的应用[J].安微农业科学,2009,37(4):1489-1492. [122] 文绍山,高必军.利用分子标记辅助选择将抗稻瘟病基因 Pi-9(t)渗入水稻恢复系泸恢 17[J].分子植物育种,2012,10(1):42-47. [123] 柏斌,吴俊,周波,等.稻瘟病抗性分子育种研究综述[J].杂交水稻, 2012,27(3):5-9. [124] 倪大虎,易成新,李莉,等.分子标记辅助培育水稻抗白叶枯病和稻瘟病三基因聚合系[J].作物学报,2008,34(1):100-105. [125] Koide Y, Kawasaki A, Telebanco Y M J, et al. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease(Magnaporthe oryzae B. Couch) in rice(Oryza sativa L.)[J]. Plant Breeding,2010,129(6):670-675. [126] 王军,杨杰,陈志德,等.利用分子标记辅助选择聚合水稻抗病基因Pi-ta、 Pi-b和 Stv-bi[J].作物学报,2011,37(6):975-981. [127] 殷得所,夏明元,李进波,等.抗稻瘟病基因 Pi9的 STS连锁标记开发及在分子标记辅助育种中的应用[J].中国水稻科学,2011,25(1): 25-30. [128] Jiang H, Feng Y, Bao L, et al. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding[J]. Molecular Breeding,2012,30:1679-1688. [129] 柳武革,王丰,刘振荣,等.利用分子标记技术聚合 Pi-1和 Pi-2基因改良三系不育系荣丰 A的稻瘟病抗性[J].分子植物育种,2012,10 (5):575-582. |
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