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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (6): 285-289.doi: 10.11924/j.issn.1000-6850.2013-1365

所属专题： 生物技术； 植物保护

• 生物技术科学 • 上一篇    下一篇

黑曲霉J6有机磷农药降解酶固定化条件的研究

王继雯 刘莹莹 李冠杰 甄静   

  • 收稿日期:2013-05-13 修回日期:2013-07-02 出版日期:2014-02-25 发布日期:2014-02-25
  • 基金资助:
    2011年省院合作项目“有机磷农药降解菌的筛选及其在土壤污染修复中的应用”(112106000027)；2012年省院合作项目“新型秸秆腐熟剂菌系筛选及开发应用研究”(122106000038)；国家自然科学基金项目“细菌脂肪酶高效表达与高通量筛选相匹配的通用系统研究”(31100582)。

Study on the Immobilization Conditions of Organic Phosphorus Pesticide Degrading Enzyme from Aspergillus niger J6

  • Received:2013-05-13 Revised:2013-07-02 Online:2014-02-25 Published:2014-02-25

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摘要： 为了制备有机磷农药水体或土壤污染修复剂，对黑曲霉J6有机磷农药降解酶的粗酶液固定化条件进行优化研究，首先制备黑曲霉J6有机磷农药降解酶的粗酶液，然后分别对海藻酸钠和CaCL2浓度以及固定化时间等固定化条件进行优化，得出其粗酶液的最佳包埋条件。研究结果表明其粗酶液最佳固定化条件为：2%海藻酸钠，4% CaCl2，包酶量20%，（其中蛋白含量为0.15 mg/mL），固定化时间4 h，在最佳条件下固定化粗酶液成球较易，比较规则，弹性好，强度较高，并且相对酶活最高。本研究成功地对黑曲霉J6有机磷农药降解酶的粗酶液的固定化条件进行了优化研究，得出了最佳包埋条件。

关键词: 物种多样性, 物种多样性

Abstract: In order to prepare remediation agent for degrading organophosphorus pesticides in polluted water or soil, immobilization conditions on the organic phosphoru pesticide degrading enzyme from Aspergillus niger J6 were studied. At first, crude enzyme liquid of organophosphorus pesticide degrading enzyme was prepared from Aspergillus niger J6, and then study on the immobilization conditions including the concentration of sodium alginate and CaCl2, and also the immobilizing time. The crude enzyme solution of the optimal entrapping condition could be gotten. The results showed that 2% sodium alginate, 4% CaCl2, the crude enzyme solution and sodium alginate solution ratio 20% (enzyme protein content is 0.15 mg/mL), the immobilizing time of 4 h, were the optimum immobilization conditions. Under the best condition, the ball was gotten more easily, better shape and elasticity, higher strength, and the relative enzyme activity reached the highest. Immobilization conditions of crude enzyme liquid from Aspergillus niger J6 were successfully studied, and the optimum embedding condition was obtained.