欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (36): 306-312.doi: 10.11924/j.issn.1000-6850.2013-1489

• 生物技术科学 • 上一篇    下一篇

高效秸秆分解菌复合系的筛选构建及性能分析

甄静 李冠杰 王继雯 刘莹莹 慕琦 杨文玲   

  • 收稿日期:2013-05-28 修回日期:2013-07-15 出版日期:2013-12-25 发布日期:2013-12-25
  • 基金资助:
    2012年省院合作项目“新型秸秆腐熟剂菌系筛选及开发应用研究”（122106000038）;2012年河南省重大科技专项“新型复合微生物肥料关键技术研究及产业化”

Selection and Construction of Composite Consortia System for Degrading Straw and Its Capability of Degradation

  • Received:2013-05-28 Revised:2013-07-15 Online:2013-12-25 Published:2013-12-25

PDF (PC)

415

摘要： 为将筛选得到的2株高产纤维素酶真菌菌株和2株高产木质素酶白腐菌菌株进行不同组配，构建出能够高效降解秸秆的复合菌系。通过测定各种组合所产的纤维素酶活和木质素酶活，根据所产酶活性的高低筛选出具有协同功能的最佳复合菌系，并研究不同pH对最佳复合菌系产酶活活性的影响。结果表明：最佳的复合菌系为球孢枝孢菌GC2-2、香菇939、毛木耳916-1，其配比组成为1:2:2，该组合所产酶活具有宽泛的pH耐受性，在偏碱性和偏酸性条件下表现出较高的酶活力。该组合具有较全面的酶学特性，这一研究工作为今后农业秸秆废弃物的应用研究奠定了一定的基础。

关键词: 因子分析, 因子分析, 线性回归, 农民收入

Abstract: To construct a composite consortia system with higher capacity of degrading straw, two high-yield cellulose-producing fungal strains were combined with two white rot fungal strains producing high-yield ligninase into different groups. Through measuring activity of cellulase and ligninase from various combinations, optimal composite consortia system with synergistic function is produced by the activity level of screening. And effects of pH on enzyme activity of the optimal composite consortia system are studied. The results showed that, the optimal composite consortia system was the combination of Cladosporium sp GC2-2, Lentinula lateritia 939 and Auricularia polytricha 916-1 in the ratio of 1:2:2. The enzyme produced by this consortium had a high tolerance for a wide pH range and showed high activity in both alkaline and acidic conditions. The composite consortia system has comprehensive enzymatic characteristics which have potential in the application of agricultural straw waste.