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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (34): 81-84.doi: 10.11924/j.issn.1000-6850.2013-1747

所属专题： 园艺； 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

番茄品种对南方根结线虫的抗性鉴定

邵颖 万景旺 朱华 黄慧文 程兆榜 周益军 魏利辉   

  • 收稿日期:2013-06-25 修回日期:2013-08-21 出版日期:2013-12-05 发布日期:2013-12-05
  • 基金资助:
    江苏省创新基金

Resistance identification against Root-Knot Nematode Meloidogyne incognita of tomato cultivars

  • Received:2013-06-25 Revised:2013-08-21 Online:2013-12-05 Published:2013-12-05

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摘要： 为了明确番茄品种对南方根据线虫的抗性情况，采用温室内盆栽番茄人工接种法， 鉴定了江苏及周边地区栽培的17个番茄品种对南方根结线虫 (Meloidogyne incognita) 的抗性程度。结果表明，天宝316表现高抗；以色列A316和粉果310表现中抗；秦樱3号、布鲁诺以及瑞星华美表现出抗病；奥米多9号、欧亚奇以及美国903表现出感病性；苏红2003、美国大红、合作903、台湾圣女、多彩水果番茄、奥利、黄贵妃樱桃番茄以及荷兰紫圣果表现出高感病性。携带Mi基因的番茄品种能够对南方根结线虫具有很好抗性，笔者利用引物SCAR F及SCAR R进行特异性检测发现，天宝(KF015736)、瑞星华美(KF015735)及多彩水果(KF015734)均获得720bp条带，与Heinz 1706（DQ437766）Mi基因同源性达到100%。

关键词: 山西省, 山西省

Abstract: In order to clearly indicated the resistance of tomato cultivars against Root-not nematode, artificial inoculation method of potted tomato plants was used in the greenhouse. Resistant degree against the Root-knot nematode, Meloidogyne incognita, of 17 cultivars of tomato cultivated in Jiangsu Province and surrounding districts was identified respectively. The results showed that cultivar of Tianbao 316 was high resistant, cultivars of Israel A316 and Fenguo310 were moderately resistant, cultivars of Qinying 3, Bruneau and RuixingHuamei were general resistant, cultivars of Aomiduo9, Ouyaqi and American 903 were susceptible, the rest cultivars that Suhong2003, AmericanDahong, Hezuo903, TaiwanShengnv, Duocaishuiguo, Aoli, Huangguifei and HollandZishengguo were highly susceptible. These cultivars, which contains gene Mi, show high level of resistance against root-knot nematode. All cultivars of tomato were detected for the resistance gene Mi by PCR used special primers SCAR F and SCAR R. The results indicated that 3 cultivars of the tomatoes carried homozygous Mi and the gene sequence of the Tiaobao (KF015736)、Ruixinghuamei(KF015735) and Duocaishuiguo(KF015734) had 100% homology with Heinz 1706（DQ437766）.