欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (31): 136-140.doi: 10.11924/j.issn.1000-6850.2014-1162

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

陕西设施蔬菜根结线虫特异性分子检测

张锋,杨苗苗,孙娟,洪波   

  1. 陕西省动物研究所,,陕西省动物研究所
  • 收稿日期:2014-04-21 修回日期:2014-04-21 接受日期:2014-09-05 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 杨苗苗
  • 基金资助:
    国家科技支撑计划“丘陵沟壑区高效农业生产关键技术集成与示范”(2011BAD31B05-04)；陕西省科学院重大科技项目“大荔现代特色农业产业保障技术研究与示范”(2014K-03)

Specific Molecular Assay for the Detection of Greenhouse Vegetables Meloidogyne spp. in Shaanxi

  • Received:2014-04-21 Revised:2014-04-21 Accepted:2014-09-05 Online:2014-11-20 Published:2014-11-20

摘要： 为明确陕西设施蔬菜根结线虫的主要种类，利用已报道的花生根结线虫[Meloidogyne arenaria (Neal.) Chitwood]、南方根结线虫[Meloidogyne incognita (Kofold & White) Chitwood]、北方根结线虫(Meloidogyne hapla Chitwood)和爪哇根结线虫[Meloidogyne javanica (Treub)]的特异性引物，对陕西省20个县市区的24个样区的根结线虫进行PCR检测。样品中检测到了南方根结线虫、北方根结线虫、花生根结线虫和爪哇根结线虫，其中南方根结线虫为优势种群，大部分样区为单一种群为害，但个别样区存在2种种群。

关键词: 对策建议, 对策建议

Abstract: In order to identify the species of vegetable root-knot nematodes in Shaanxi Province，the reported specific primers of Meloidogyne arenaria (Neal.) Chitwood, Meloidogyne incognita (Kofold & White) Chitwood, Meloidogyne hapla Chitwood and Meloidogyne javanica (Treub) were served as polymerase chain reaction (PCR) assay primers respectively to detect Meloidogyne spp. of greenhouse vegetables that obtained from twenty four sampling areas that distributed in twenty cities and counties in Shaanxi. Our study showed that M. arenaria, M. incognita, M. hapla and M. javanica were detected, and M. incognita was the dominant species; most sampling areas were infected by single population, however, specific sampling areas were infected by two populations.