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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (30): 273-277.doi: 10.11924/j.issn.1000-6850.2014-1435

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

湖水中低温菌的筛选及其产酶特性

勾长龙   

  1. 吉林农业大学
  • 收稿日期:2014-05-18 修回日期:2014-05-18 接受日期:2014-08-08 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 勾长龙
  • 基金资助:
    国家现代农业产业技术体系建设专项“牛场环境控制与废弃物处理技术”（CARS-38）；2013 年公益性行业（农业）科研专项“牛场废弃物处 理利用技术模式集成与示范”（201303091）

Isolation and Identification of Psychrophile from the Lake and the Properties of Enzyme Production

  • Received:2014-05-18 Revised:2014-05-18 Accepted:2014-08-08 Online:2014-10-31 Published:2014-10-31

摘要： 为了探索湖水中低温微生物及其酶活的多样性，并获得具有应用价值的低温菌。以低温环境下湖水为样品，通过平板涂布法和划线分离法对其进行初步分离纯化，进一步进行菌落形态、微观形态观察以及生理生化性质的测定，筛选出能够在低温下生长的菌株。并进行蛋白酶、纤维素酶、淀粉酶、木质素酶功能的测定。结果显示共筛选出可培养的低温微生物22 株，包括细菌14 株，真菌5 株，放线菌3株。7 株具有蛋白酶活性，3 株具有淀粉酶活性，6 株具有木质素酶活性，6 株具有纤维素酶活性。其中WF-1 具有四种酶活性，WB-8 具有三种酶活性，其他菌株具有一种或者两种酶活力。筛选的低温菌株具有多种酶活性，具有潜在的开发价值。

关键词: pH值, pH值

Abstract: The study aims to explore microbial and enzyme activity diversity of the lake, and obtain the cold bacteria with application value. The samples from the lake at low temperature were taken and spread onto plate for separating. The strains were further measured by colony morphology, physiological and biochemical properties, screened out the cold- adapt strains. The protease, cellulose, amylase, and the lignin enzyme activity of strains were also measured. The results showed that the total of 22 cold-adapted-strains were isolated, which contained 14 bacteria, 5 fungi and 3 actinomyces. Seven of them can produce protease, three of them can produce amylase, six of them can produce lignin enzymes, six of them can produce cellulase. WF-1 has four enzyme activities, WB- 8 has three enzyme activities, the other strains have one or two enzyme activities. The screened out strains have various enzyme activities and potential value for development.