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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 40-44.doi: 10.11924/j.issn.1000-6850.2014-1970

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

不同品种橡胶树花药愈伤组织诱导、分化及植株再生的比较

分化及植株再生的比较   

  1. 管 艳,李 玲,梁国平,桂明春,黄风翔,王 亚,田 海(云南省热带作物科学研究所,云南景洪 666100）
  • 收稿日期:2014-07-17 修回日期:2015-01-29 接受日期:2014-12-12 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 分化及植株再生的比较
  • 基金资助:
    国家科技支撑计划项目“橡胶树自根幼态无性系繁殖技术的开发应用”(2011BAD30B01)；云南省产业重大关键技术开发专项计划“橡胶树优良品种体胚植株培养和籽苗繁殖关键技术集成与开发”[云发改(2008)1658号]；景洪市科技三项费项目“橡胶树优良品种体胚再生体系建立的研究”[(2014)45号]；云南省热带作物科技创新体系建设资金“橡胶树育种材料及方法创新”(RF2014)；云南省科研院所技术开发研究专项“橡胶树优良品种组培快繁和籽苗芽接配套育苗技术研究与集成示范”(2013DC013)。

Comparison of Callus Induction, Somatic Embryogenesis and Plant Regeneration

from Different Clones of Hevea brasiliensis Anther   

  1. Guan Yan, Li Ling, Liang Guoping, Gui Mingchun, Huang Fengxiang, Wang Ya, Tian Hai(Yunnan Tropical Crops Research Institute, Jinghong Yunnan 666100)
  • Received:2014-07-17 Revised:2015-01-29 Accepted:2014-12-12 Online:2015-03-20 Published:2015-03-20

摘要： 为优化橡胶树花药体细胞胚诱导及植株再生体系，为橡胶树分子育种提供技术支撑，以橡胶树品种‘云研73-477’、‘云研73-46’、‘热研8-79’、‘云研77-4’、‘RRII105’、‘热垦525’的花药为材料，比较不同品种在相同培养条件下愈伤组织诱导、体胚发生能力及植株再生频率。结果表明，6个品种的花药均能脱分化形成愈伤组织，但不同品种间存在显著差异，诱导率最高的是‘RRII105’和‘热垦525’，分别为93.33%、90.33%，最低的是‘云研77-4’，为31.33%；体胚发生能力在不同品种间也有较大差异，‘云研73-477’体胚诱导率最高达76%，‘云研73-46’没有分化出体细胞胚；植株再生频率较高的品种有‘热垦525’和‘云研73-477’，分别为75.38%、54.35%，‘云研73-46’和‘云研77-4’无再生植株。

关键词: 转录间隔区, 转录间隔区

Abstract: In order to optimize the somatic embryogenesis and plant regeneration system of anther and provide a molecular breeding protocol for Hevea brasiliensis, callus induction, somatic embryogenesis and plant regeneration were compared under the same condition using anthers from six clones ‘Yunyan 73-477’, ‘Yunyan 73-46’, ‘Reyan 8-79’, ‘Yunyan 77-4’, ‘RRII105’ and ‘Reken 525’ of H. brasiliensis. The results indicated that the anthers from six clones were all able to dedifferentiate into callus tissues. But there were significant differences among different clones, ‘RRII105’ and ‘Reken 525’ showed the highest callus induction rates of 93.33% and 90.33%, respectively. The lowest differentiation rate was 31.33%, which was found in ‘Yunyan 77-4’. The somatic embryogenesis of different clones was significantly different, the highest differentiation rate was 76% which was found in ‘Yunyan 73-477’, and the clones of ‘Yunyan 73-46’ had no somatic embryo. ‘Reken 525’ and ‘Yunyan 73-477’ showed high plant regeneration rates of 75.38% and 54.35%, respectively, the clones of ‘Yunyan 73-46’ and ‘Yunyan 77-4’ had no regeneration plant.