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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 6-11.doi: 10.11924/j.issn.1000-6850.2013-0616

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

芭蕉属rDNA的ITS序列分析

王静毅 陈雪婷 武耀廷   

  • 收稿日期:2013-03-07 修回日期:2013-05-05 出版日期:2013-09-05 发布日期:2013-09-05

Analysis of Nuclear Ribosomal ITS Sequences in Musa (Musaceae)

  • Received:2013-03-07 Revised:2013-05-05 Online:2013-09-05 Published:2013-09-05

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摘要： 为了研究芭蕉属ITS序列的变异情况和组群关联，以不同地理来源的33份芭蕉属野生种作为试材，对其ITS区进行扩增、测序和序列比对，构建NJ系统树。结果表明：芭蕉属中不同类型材料的ITS序列信息位点为108个，占总碱基数的24.43%，具有较强的系统发育信号；组群间序列比对结果分析发现澳蕉组ITS序列与其他组别相比具有非常显著的差异，特异性片段共21处；从序列上推断澳蕉组可能由M.schizocarpa与M.balbisiana(ITC0271)杂交产生；真蕉组和观赏蕉组没有确切的分类特性；ITS序列的分化上可能受到地理分布的影响；陈友鉴定的新种M.tongbiguanensi在ITS序列上与其他种可明显区分开，从分子水平上验证了新种的可能性。利用核糖体ITS序列对进一步深入研究芭蕉属植物遗传演化关系具有一定的指导意义。

关键词: 净化, 净化

Abstract: In order to analyze the variations of ITS sequences in Musa, the ITS sequences of 33 wild species from different geographical origin in Musa were amplified by PCR and sequenced, and then the NJ tree was constructed by ITS sequences. The results showed that, the sequences of the ITS in Musa had a strong phylogenetic signal. 108 (24.43%) informative base sites were detected. And a lot of indels and/or single base substitutions were observed in the sequences of various samples in Sect. Australimusa, which had 21 different sequence sites from other sections. According to the analysis of the sequences, we could infer that Sect. Australimusa might be the inter-specific hybrids between M. schizocarpa and M. balbisiana (ITC0271). Sect. Eumusa and Sect. Rhodochlamys had no difference in the sequences. The ITS sequence differentiation was possibly affected from the influence of the geographic distribution. A new species, M.tongbiguanensi identified by Chen, could be distinguished from other species in ITS sequence. It proved that M.tongbiguanensi might be a new species in molecular level. The sequences analysis of ITS was good to discriminate the phylogenetic relation between species/subspecies in Musa.