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中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 182-186.doi: 10.11924/j.issn.1000-6850.casb14120005

• 植物保护 农药 • 上一篇    下一篇

一种室内大量饲养灰飞虱的方法

任春梅,程兆榜,杨柳,缪倩,周益军   

  1. 江苏省农业科学院植物保护研究所,江苏省农业科学院植物保护研究所,江苏省农业科学院植物保护研究所,江苏省农业科学院植物保护研究所,江苏省农业科学院植物保护研究所
  • 收稿日期:2014-12-01 修回日期:2015-02-15 接受日期:2015-03-03 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 程兆榜
  • 基金资助:
    国家自然科学“灰飞虱地理种群的标记研究”(31201506);江苏省农业科技自主创新项目“灰飞虱地理种群的标记研究”[CX(13)5026]。

A Method for Mass Rearing of Small Brown Planthopper (Laodelphax striatellus ) in Laboratory

  • Received:2014-12-01 Revised:2015-02-15 Accepted:2015-03-03 Online:2015-06-02 Published:2015-06-02

摘要: 灰飞虱是重要的农业害虫和病毒传播介体,为给相关研究提供足够的活体材料,建立了一种室内灰飞虱规模化饲养的方法,笔者经一系列生物学试验,对温度、光强、饲养密度等条件进行了优化,并对产卵和换苗时间进行了规定。试验表明饲养温度24~28℃,光照强度5000~7000lx,不同龄期虫的饲养密度分别为300~400头/杯(若虫)、200~300头/杯(3~4龄虫)、100~200头/杯(成虫)适合各自饲养时水稻苗的生长、灰飞虱的发育繁殖、卵的孵化等。为保证同批次虫龄的一致性,规定产卵时间和换苗时间分别为48h和8~10天。此方法条件因素明确,可操作性强,不受场地、时间、环境等自然条件限制;可随时提供足量试验用龄次标准试虫。

关键词: 库尔勒香梨, 库尔勒香梨, UV-C, 1-MCP, 采后品质, 生理活性

Abstract: Small brown planthoppers (Laodelphgax striatellus) are agricultural pests and mediators to spread plant virus. In order to provide sufficient insect materials in vivo for correlational research, a method for mass rearing of small brown planthopper in laboratory was presented. We optimized the temperature, light intensity, rearing density and fixed the time of laying eggs, changing seedlings through a series of biological tests. Results showed that temperature of 24-28℃, light intensity of 5000-7000 lx, rearing density of 300-400 SBPH per beaker (nymph), 200-300 SBPH per beaker (3th-4th instar), and 100-200 SBPH per beaker (adults) were the best condition for rice growing, SBPH development, breeding and hatching eggs of each rearing batch. For the consistency of instars between different batches, the times of 48 hours and 8 to 10 days were respectively fixed for laying eggs and changing seedlings. Factors of rearing condition were explicit using this method, and were easy to operate. It was also not limited to space, time, environment and other natural conditions and could provide enough insects at standard experimental age at any time.