关键词: 猪流行性腹泻病毒, 猪流行性腹泻病毒, SYBR Green I, Real-time PCR, 定量检测

Abstract: In order to know scientific efficacy of nitrogen stabilizer and apply this kind of product in Jiangnan band region, we explored its field performance on winter wheat. Based on the same use rate of Phosphate and Potash fertilizer, we studied the effect of nitrogen stabilizer on wheat yield, agronomic characteristics, nitrogen agronomic efficacy and fertilization benefit under different application methods. Trial results showed that yield performance of treatment with nitrogen could increase 3075- 4410 kg/hm² compared with untreated, the increase rate at least reached 88.9%. Treatment with nitrogen stabilizer averagely increased productive ear 1 million/hm², the increase rate could reach 15.61% . The average yield could increase to 528.25 kg/hm² and increase rate was 7.74%. Nitrogen agronomic efficacy averagely increases 3 kg/kg and the increase rate was 16.75% . Decreasing 25% of nitrogen with nitrogen stabilizer had the highest wheat product efficacy, which could reach 25.36 kg/kg. Treatment 50% nitrogen as base fertilizer mixed with nitrogen stabilizer following other 50% nitrogen as side- dress fertilizer had the highest yield and income results, which could reach 7869 kg/hm² and 8524.50 yuan/hm² respectively. Based on our trial results, nitrogen was the most important factor on wheat growth under this trial soil fertility. Nitrogen stabilizer facilitated the increase of productive ear number and percentage of ear bearing tiller. Nitrogen stabilizer increased the utilization of nitrogen and productivity of wheat, which turned out to save fertilizer use rate and cost. Application 50% nitrogen as base fertilizer mixed with nitrogen stabilizer following other 50% nitrogen as side-dress fertilizer is recommended.