动物源性食品中猪源性成分检测

doi:10.11924/j.issn.1000-6850.casb15080002

中国农学通报 ›› 2015, Vol. 31 ›› Issue (36): 259-264.doi: 10.11924/j.issn.1000-6850.casb15080002

所属专题：畜牧兽医

动物源性食品中猪源性成分检测

陈正芳¹,曾萍¹,杨具田¹,臧荣鑫¹,曹忻²,徐红伟²

(¹西北民族大学生命科学与工程学院,兰州730030；²西北民族大学试验中心,兰州 730030）

收稿日期:2015-08-01 修回日期:2015-09-18 接受日期:2015-09-25 出版日期:2015-12-30 发布日期:2015-12-30
通讯作者: 徐红伟
基金资助:
2014西北民族大学本科生科研创新项目“SYBR Green I Real time PCR对牛、羊、鸡、马、鸭、猪源性成分定性、定量分析研究”(URIP14149)。

Detection of Swine Derived Ingredients in Animal Derived Foods

Chen Zhengfang¹, Zeng Ping¹, Yang Jutian¹, Zang Rongxin¹, Cao Xin², Xu Hongwei²

(¹College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030;²Scientific Experimental Center, Northwest University for Nationalities, Lanzhou 730030)

Received:2015-08-01 Revised:2015-09-18 Accepted:2015-09-25 Online:2015-12-30 Published:2015-12-30

摘要/Abstract

摘要： 为探讨猪源性成分检测可行的方法，利用SYBR Green I Real time PCR对动物源性食品中猪源性成分进行测定。根据猪mtDNA保守序列设计特异性引物，对牛、羊、猪的动物源性食品进行扩增；克隆目的基因片段，并以此为标准品，优化反应条件和反应体系，提高灵敏度；反复检测不同样品，测试稳定性。结果表明SYBR Green I Real time PCR技术能够特异性的检测到动物源性食品中猪源性成分，引物特异性强、稳定性可靠，灵敏度达到1×10^-6 ng/μL，建立了动物源性食品中猪源性成分的快速、精准、经济、便捷的检测方法。

关键词: 比较优势, 比较优势, 畜产品, 水产品, 环渤海

Abstract: To establish a specific method detecting the swine derived ingredients in some animal derived foods, a real-time PCR was established according to the conserved sequences of swine mtDNA, specific primers were designed and the animal derived products of bovine, ovine and swine were amplified. The target genes were cloned and reaction system was optimized to improve detection sensitivity. Different samples were tested repeatedly to test the stability of the detection method. The results demonstrated that only swine derived ingredients in those animal derived foods were detected. And the detection sensitivity reached 1×10^-6 ng/μL. The established method was excellent for identification of swine derivatives in animal derived foods as it was accurate, cheap, convenient and fast.

中图分类号:

TS251.7

陈正芳,曾萍,杨具田,臧荣鑫,曹忻,徐红伟. 动物源性食品中猪源性成分检测[J]. 中国农学通报, 2015, 31(36): 259-264.

Chen Zhengfang,Zeng Ping,Yang Jutian,Zang Rongxin,Cao Xin and Xu Hongwei. Detection of Swine Derived Ingredients in Animal Derived Foods[J]. Chinese Agricultural Science Bulletin, 2015, 31(36): 259-264.

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动物源性食品中猪源性成分检测

Detection of Swine Derived Ingredients in Animal Derived Foods

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