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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (2): 51-56.doi: 10.11924/j.issn.1000-6850.casb16040102

所属专题： 园艺； 农业气象

• 林学 园艺 园林 • 上一篇    下一篇

基于隶属函数法的PEG-6000模拟干旱下 文山辣椒抗氧化能力分析

赵水灵,孟凡来,赵昶灵,屈用函,袁恩平,杨羚钰,张雪廷,王绍祥,李 云   

  1. 文山农业科学院,文山农业科学院,云南农业大学农学与生物技术学院,文山农业科学院,文山农业科学院,文山农业科学院,文山农业科学院,文山农业科学院,文山农业科学院
  • 收稿日期:2016-04-17 修回日期:2016-12-27 接受日期:2016-05-25 出版日期:2017-02-09 发布日期:2017-02-09
  • 通讯作者: 赵昶灵
  • 基金资助:
    ：云南省对外科技合作计划项目“邹学校专家工作站”(2015IC057)；云南省科技创新强省计划项目“文山小米辣产业化关键技术研究及集成 示范”(2015AB011)；文山农业科学院资助项目“‘丘北彩色辣椒’在文山生境的抗旱性研究”(HZ2015-01)。

Antioxidant Capacity of Capsicum annuum Cultivars in Wenshan to the Drought Stress Simulated by PEG-6000 Based on Subordinate Function

  • Received:2016-04-17 Revised:2016-12-27 Accepted:2016-05-25 Online:2017-02-09 Published:2017-02-09

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摘要： 为了评价云南文山州主栽辣椒品种在干旱胁迫下的抗氧化能力及其与品种紫化程度的关联性，用分光光度法研究了云南文山州主栽辣椒品种‘ZSZ75-1’、‘ZSZ49-1-1’、‘12WS-18-1’、‘12ZH01’和‘ZS130’叶片在PEG-6000 模拟干旱胁迫下抗氧化酶比活力和丙二醛(MDA)含量，并用隶属函数法分析了各品种叶片的综合抗氧化能力。结果表明：在PEG-6000 模拟干旱胁迫下，5 个辣椒品种叶片可溶性蛋白质含量特征为：‘ZSZ75-1’＞‘12ZH01’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘ZS130’，过氧化氢酶(CAT)比活力为：‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘ZS130’＞‘12WS-18-1’＞‘12ZH01’，过氧化物酶(POD)比活力为：‘ZSZ75-1’＞‘ZS130’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘12ZH01’，超氧化物歧化酶(SOD)比活力为：‘12WS-18-1’＞‘ZS130’＞‘12ZH01 ’＞‘ZSZ49-1-1’＞‘ZSZ75-1’，MDA 含量为：‘ZSZ75-1’＞‘12WS-18-1’＞‘12ZH01’＞‘ZS130’＞‘ZSZ49-1-1’。但是，5 个品种叶片可溶性蛋白质和MDA含量及CAT和SOD比活力之间的差异均未达到显著水平，而POD比活力间的差异均仅达到显著水平。5 个品种叶片的综合抗氧化能力为：‘ZS130’＞‘ZSZ49-1-1’＞‘ZSZ75-1’＞‘12WS-18-1’＞‘12ZH01’。因此，文山州主栽辣椒品种特定器官积累花色苷并不贡献于叶片在干旱胁迫下抗氧化能力的强化。本研究可为文山州辣椒品种抗旱能力的评价、花色苷积累和抗旱性关系的探究和抗旱新品种的选育提供参考。

关键词: 云顶亮温TBB, 云顶亮温TBB, 大暴雨, 中尺度系统, 干冷空气

Abstract: In order to evaluate the antioxidant capacity of the main Capsicum annuum cultivars cultivated in Wenshan Prefecture, Yunnan Province under drought stresses and its relationship with the purpling degrees of the cultivars, the specific activities of antioxidases and content of malondialdehyde (MDA) in the leaves of the five main C. annuum cultivars including ‘ZSZ75-1’, ‘ZSZ49-1-1’, ‘12WS-18-1’, ‘12ZH01’ and ‘ZS130’ cultivated in Wenshan were studied by using spectrophotometry under the drought stress simulated by PEG-6000, and the comprehensive antioxidant capacity of the leaves was evaluated by using subordinate function. The results showed that under the drought stress simulated by PEG-6000, the content of soluble protein in the leaves of the five cultivars was ‘ZSZ75-1’＞‘12ZH01’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘ZS130’, the specific activity of catalase (CAT) was ‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘ZS130’＞‘12WS-18-1’＞‘12ZH01’, the specific activity of peroxidase (POD) was ‘ZSZ75-1’＞‘ZS130’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘12ZH01’, the specific activity of superoxide dismutase (SOD) was ‘12WS-18-1’＞‘ZS130’＞‘12ZH01’＞‘ZSZ49-1-1’＞‘ZSZ75-1’, and the content of MDA was ‘ZSZ75-1’＞‘12WS-18-1’＞‘12ZH01’＞‘ZS130’＞‘ZSZ49-1-1’. However, the differences of the contents of the soluble protein and MDA and the specific activities of CAT and SOD of the five cultivar leaves were not significant, whereas the difference of POD specific activity was siginificant. The comprehensive antioxidant ability of the five cultivars was ‘ZS130’＞‘ZSZ49-1-1’＞‘ZSZ75-1’＞‘12WS-18-1’＞‘12ZH01’. Thus, the anthocyanin accumulation in the specific organs of the main C. annuum cultivars in Wenshan does not contribute to the strengthening of the leaf antioxidant capacity under drought stress. This study could provide a reference for the drought resistance evaluation, the exploration of the relationship between the anthocyanin accumulation and drought resistance, and the selection of the new drought resistant cultivars of C. annuum in Wenshan.

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