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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (33): 147-150.doi: 10.11924/j.issn.1000-6850.casb16050175

• 生物技术科学 • 上一篇    下一篇

大孔吸附树脂对药桑多糖的脱色及抗氧化活性研究

龚敏,赵德前,孙慧洁   

  1. 海南省现代农业检验检测预警防控中心,石河子大学 化学化工学院,海南省现代农业检验检测预警防控中心;石河子大学 化学化工学院
  • 收稿日期:2016-05-30 修回日期:2016-10-25 接受日期:2016-07-25 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 龚敏
  • 基金资助:
    无

Effect of Macroporous Resin on Decolorization and Antioxidant Activity of Polysaccharide from Morus nigra Linn

  • Received:2016-05-30 Revised:2016-10-25 Accepted:2016-07-25 Online:2016-11-25 Published:2016-11-25

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摘要： 研究大孔吸附树脂对药桑多糖脱色工艺和脱色前后多糖抗氧化活性变化的影响。采用静态吸附法选出对药桑多糖具有最佳脱色效果的树脂，动态吸附法系统地研究吸附工艺条件，采用DPPH· 自由基法对脱色前后多糖抗氧化活性进行对比研究。NKA-9 型大孔树脂对药桑多糖的脱色效果最佳。药桑多糖脱色率可达61.8%，最佳脱色条件为：流速：4.8 BV/h，多糖浓度：3.261 mg/mL，洗脱剂浓度：60%。在该工艺条件下，药桑多糖的脱色率可达到最大，脱色后多糖抗氧化活性有所增强。

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Abstract: The aim was to study the effect of macroporous resins on the decoloring process of crude polysaccharide extracted from Morus nigra Linn and the antioxidant activity of polysaccharide before and after decoloring. The static adsorption method was used to select the best decolorization resin; the dynamic adsorption method was employed to systematically investigate the absorption process conditions; and the comparative study of antioxidant activity of crude and decolorant polysaccharide was conducted by DPPH. The results showed that resin NKA- 9 provided the best decolorizing property, and the decolorization rate was 61.8%. The optimum decoloring condition of NKA-9 was as follows: the flow rate 4.8 BV/h, the polysaccharide concentration 3.261 mg/mL, and the eluent concentration 60%. Under this condition, the decolorization rate could be maximized, and the cellular antioxidant activity of decolorant polysaccharide was enhanced after decoloring by NKA-9.