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中国农学通报 ›› 2017, Vol. 33 ›› Issue (25): 52-58.doi: 10.11924/j.issn.1000-6850.casb17040082

• 林学 园艺 园林 • 上一篇    下一篇

4种植物根系活性保存方法及保存时间初探

李雪松,刘静   

  1. 内蒙古农业大学沙漠治理学院730,内蒙古农业大学沙漠治理学院730
  • 收稿日期:2017-04-13 修回日期:2017-08-08 接受日期:2017-07-11 出版日期:2017-09-15 发布日期:2017-09-15
  • 通讯作者: 刘静
  • 基金资助:
    国家自然科学基金资助项目“根系固土抗蚀受损后自我修复的力学特性”(51364034)。

Root Activity of 4 Plant Species: Preservation Method and Storage Time

  • Received:2017-04-13 Revised:2017-08-08 Accepted:2017-07-11 Online:2017-09-15 Published:2017-09-15

摘要: 研究根系的材料力学特性时,根系一般处于离体状态,目前对于柠条、沙柳、沙棘、白沙蒿4种植物根系离体后的存活时间和存活状态的研究及保存方法的探讨还未见报道,所以确定植物根系材料力学特性研究的适宜保存时间及保存方法亟待解决。本研究采用TTC-脱氢酶还原法测定1~4mm径级范围内的上述4种植物根系在4℃低温培养箱中保存0天、6天、13天、20天的根系活性值,结果表明:4℃低温培养箱更适合较长时间保存植物根系活性;4种植物的根系活性减小率均随保存时间的增加而快速增长,当保存6天时,4种植物的根系活性减小率达到10%~20%,当保存20天时,4种植物的根系活性减小率达到70%~90%;保存时间相同时,在1~4mm根径范围内,4种植物基本均随根径的增加根系活性减小率变小,即细根的根系活性更易损失。

关键词: 蓝莓园土壤, 蓝莓园土壤, pH值, 主要养分, 改良对策

Abstract: When studying the mechanical properties of the roots, the roots are generally in the isolated state. At present, there is no reports on the survival time and survival status of 4 species of Caragana korshinskii Kom, Salix psammophila Schneid, Hippophae rhamnoides Linn, Artemisia sphaerocephala Krasch in vitro. Therefore, it is urgent to determine the suitable storage time and the preservation method for the study of the mechanical properties of plant roots. In this research, TTC- dehydrogenase reduction method was used to determine the root activity of 4 kinds of plant roots in the 1~4 mm diameter range in the low temperature incubator of 4℃ for 0 days, 6 days, 13 days and 20 days. The results showed that: low temperature incubator of 4℃ was more suitable for the preservation of plant root activity for a long time, the root activity of 4 kinds of plants was the rapid growth rate of decrease with increasing storage time, when stored for 6 days, the root activity of 4 plants decreased at a rate of 10%~20%, when stored for 20 days, the root activity of 4 species decreased at a rate of 70%~90%; When the storage time was the same, the root activity decreased with the increase of root diameter in the range of 1~4 mm root diameter, and the root activity of fine roots was more likely to be lost in the 4 plants.

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