氟雷拉纳与4种杀虫剂对斜纹夜蛾的室内毒力及其联合作用

doi:10.11924/j.issn.1000-6850.casb18120032

摘要/Abstract

摘要：

为明确氟雷拉纳对斜纹夜蛾Spodoptera litura Fabricius的毒力及其与毒死蜱、茚虫威、溴氰菊酯、甲氨基阿维菌素苯甲酸盐（简称：甲维盐）复配后的联合作用,筛选出最佳配比,从而为其混剂研发提供科学依据。笔者采用室内人工饲料混毒法,测定了氟雷拉纳与毒死蜱、茚虫威、溴氰菊酯、甲维盐单剂及其混剂对斜纹夜蛾三龄幼虫的毒力,并通过共毒系数法评价复配药剂的联合作用。单剂结果表明：氟雷拉纳、溴氰菊酯和甲维盐对斜纹夜蛾具有高毒力,药后48 h的LC₅₀值分别为0.48、0.65、0.38 mg/kg;毒死蜱和茚虫威对斜纹夜蛾的毒力中等,药后的LC₅₀值分别为8.71、3.08 mg/kg。混配结果表明：氟雷拉纳与毒死蜱混配具有良好的增效作用,且配比为3:2时,共毒系数最高为164.2;与茚虫威混配时,具有相加作用;而与溴氰菊酯或甲维盐混配时,却均表现为拮抗作用。总之,本研究为氟雷拉纳的增效混剂研发提供了科学依据;而因其与溴氰菊酯或甲维盐混配存在拮抗作用,应避免用于混剂研发。

关键词: 杀虫剂, 斜纹夜蛾, 氟雷拉纳, 农药混配, 共毒系数

Abstract:

To identify the toxicity of fluralaner and its joint action with four traditional insecticides (chlorpyrifos, indoxacarb, deltamethrin and emamectin benzoate) to Spodoptera litura F. and explore the optimum ratio of insecticides, the 3rd instar larvae of S. litura and artificial food mixing insecticides were used in the study, the toxicity was measured and the joint action was evaluated by co-toxicity coefficient. The results of single dosage showed that fluralaner, deltamethrin and emamectin benzoate exhibited high activity with LC₅₀ values of 0.48, 0.65 and 0.38 mg/kg at 48 h, respectively. Meanwhile, chlorpyrifos and indoxacarb exhibited moderate activity to S. litura with LC₅₀ values of 8.71 and 3.08 mg/kg, respectively. The results of joint action showed that fluralaner mixing with chlorpyrifos at 3:2, the mixture showed the highest synergistic effect with co-toxicity coefficient (CTC) as 164.2, the mixture with indoxacarb showed additive effect, the mixture with deltamethrin or emamectin benzoate showed antagonistic effect. In conclusion, these results demonstrate that fluralaner and chlorpyrifos could be considered as effective insecticidal mixture to be used for control of S. litura. On the contrary, the fluralaner and deltamethrin or emamectin benzoate should avoid combined application due to their antagonism.

Key words: insecticide, Spodoptera litura F., fluralaner, insecticide mixture, co-toxicity coefficient

中图分类号:

S482.3

刘迪, 布琼, 唐涛, 凌汉, 赵春青. 氟雷拉纳与4种杀虫剂对斜纹夜蛾的室内毒力及其联合作用[J]. 中国农学通报, 2020, 36(9): 113-116.

Di Liu, Qiong Bu, Tao Tang, Han Ling, Chunqing Zhao. Toxicity and Joint Action of Fluralaner and Four Insecticides Against Spodoptera litura Fabricius[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 113-116.

图/表 2

参考文献 22

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供试药剂	致死中浓度LC₅₀/(mg/kg)	95%置信限	斜率±标准误	回归方程	相关系数
氟雷拉纳	0.48	0.41~0.54	3.711±0.454	y=0.47+3.87x	0.964
毒死蜱	8.71	8.16~9.19	8.432±1.353	y=-8.52+8.64x	0.917
茚虫威	3.08	2.61~3.56	4.359±0.575	y=-3.63+4.31x	0.980
溴氰菊酯	0.65	0.40~1.29	3.261±0.487	y=0.33+2.59x	0.957
甲维盐	0.38	0.12~0.83	3.407±0.451	y=0.14+2.01x	0.864

供试药剂	致死中浓度LC₅₀/(mg/kg)	95%置信限	斜率±标准误	回归方程	相关系数
氟雷拉纳	0.48	0.41~0.54	3.711±0.454	y=0.47+3.87x	0.964
毒死蜱	8.71	8.16~9.19	8.432±1.353	y=-8.52+8.64x	0.917
茚虫威	3.08	2.61~3.56	4.359±0.575	y=-3.63+4.31x	0.980
溴氰菊酯	0.65	0.40~1.29	3.261±0.487	y=0.33+2.59x	0.957
甲维盐	0.38	0.12~0.83	3.407±0.451	y=0.14+2.01x	0.864

混配名称	配比	回归方程	决定系数	LC₅₀<break/>(95%置信限)	实际毒性<break/>指数(ATI)	理论毒性<break/>指数(TTI)	共毒系数<break/>(CTC)	评价
氟雷拉纳：毒死蜱	1:4	y=-0.57+2.41x	0.988	1.70(1.38~1.99)	28.2	24.4	115.7	相加
	2:3	y=0.15+2.18x	0.883	0.85(0.43~1.10)	56.5	43.3	130.4	增效
	3:2	y=0.75+2.31x	0.980	0.47(0.34~0.59)	102.1	62.2	164.2	增效
	4:1	y=0.92+2.96x	0.874	0.65(0.50~0.77)	73.8	81.1	91.1	相加
氟雷拉纳：茚虫威	1:4	y=-0.33+4.80x	0.992	1.15(1.00~1.31)	41.7	32.5	128.6	增效
	2:3	y=-0.04+4.09x	0.937	1.05(0.90~1.22)	45.3	49.4	92.6	相加
	3:2	y=0.28+4.29x	0.991	0.80(0.48~1.03)	60.0	66.2	90.6	相加
	4:1	y=0.90+4.65x	0.981	0.64(0.52~0.75)	75.0	83.1	90.2	相加
氟雷拉纳：溴氰菊酯	1:4	y=-0.33+3.79x	0.991	1.21(1.03~1.40)	39.7	79.1	50.2	拮抗
	2:3	y=-0.34+4.23x	0.812	1.27(0.48~2.91)	37.8	84.3	44.8	拮抗
	3:2	y=-1.36+4.11x	0.948	2.20(1.91~2.62)	21.8	89.5	24.4	拮抗
	4:1	y=-1.17+3.89x	0.942	2.05(1.73~2.34)	23.4	94.8	24.7	拮抗
氟雷拉纳：甲维盐	1:4	y=-0.02+2.98x	0.922	1.00(0.81~1.19)	48.0	121.1	39.7	拮抗
	2:3	y=-0.78+3.76x	0.991	1.61(1.39~1.88)	29.8	115.8	25.7	拮抗
	3:2	y=-0.15+2.65x	0.983	1.16(0.92~1.41)	41.4	110.5	37.4	拮抗
	4:1	y=-0.02+3.57x	0.950	1.01(0.52~1.51)	47.5	105.3	45.1	拮抗

混配名称	配比	回归方程	决定系数	LC₅₀<break/>(95%置信限)	实际毒性<break/>指数(ATI)	理论毒性<break/>指数(TTI)	共毒系数<break/>(CTC)	评价
氟雷拉纳：毒死蜱	1:4	y=-0.57+2.41x	0.988	1.70(1.38~1.99)	28.2	24.4	115.7	相加
	2:3	y=0.15+2.18x	0.883	0.85(0.43~1.10)	56.5	43.3	130.4	增效
	3:2	y=0.75+2.31x	0.980	0.47(0.34~0.59)	102.1	62.2	164.2	增效
	4:1	y=0.92+2.96x	0.874	0.65(0.50~0.77)	73.8	81.1	91.1	相加
氟雷拉纳：茚虫威	1:4	y=-0.33+4.80x	0.992	1.15(1.00~1.31)	41.7	32.5	128.6	增效
	2:3	y=-0.04+4.09x	0.937	1.05(0.90~1.22)	45.3	49.4	92.6	相加
	3:2	y=0.28+4.29x	0.991	0.80(0.48~1.03)	60.0	66.2	90.6	相加
	4:1	y=0.90+4.65x	0.981	0.64(0.52~0.75)	75.0	83.1	90.2	相加
氟雷拉纳：溴氰菊酯	1:4	y=-0.33+3.79x	0.991	1.21(1.03~1.40)	39.7	79.1	50.2	拮抗
	2:3	y=-0.34+4.23x	0.812	1.27(0.48~2.91)	37.8	84.3	44.8	拮抗
	3:2	y=-1.36+4.11x	0.948	2.20(1.91~2.62)	21.8	89.5	24.4	拮抗
	4:1	y=-1.17+3.89x	0.942	2.05(1.73~2.34)	23.4	94.8	24.7	拮抗
氟雷拉纳：甲维盐	1:4	y=-0.02+2.98x	0.922	1.00(0.81~1.19)	48.0	121.1	39.7	拮抗
	2:3	y=-0.78+3.76x	0.991	1.61(1.39~1.88)	29.8	115.8	25.7	拮抗
	3:2	y=-0.15+2.65x	0.983	1.16(0.92~1.41)	41.4	110.5	37.4	拮抗
	4:1	y=-0.02+3.57x	0.950	1.01(0.52~1.51)	47.5	105.3	45.1	拮抗