根腐病菌侵染对黄芪苯丙烷途径关键酶活性的影响

doi:10.11924/j.issn.1000-6850.casb20190700367

中国农学通报 ›› 2020, Vol. 36 ›› Issue (25): 115-120.doi: 10.11924/j.issn.1000-6850.casb20190700367

所属专题：生物技术；园艺

根腐病菌侵染对黄芪苯丙烷途径关键酶活性的影响

赵雪姣¹^,²(), 秦雪梅¹, 王梦亮³, 高芬³()

¹山西大学中医药现代研究中心,太原 030006
²山西大学化学化工学院,太原 030006
³山西大学应用化学研究所,太原 030006

收稿日期:2019-07-02 修回日期:2019-11-05 出版日期:2020-09-05 发布日期:2020-08-18
通讯作者: 高芬
作者简介:赵雪姣,女,1994年出生,湖北恩施人,在读硕士,研究方向：根腐病菌与黄芪的互作。通信地址：030006 山西省太原市小店区坞城路92号山西大学应用化学研究所,E-mail： 1601009095@qq.com。
基金资助:
山西省应用基础研究项目“蒙古黄芪响应根腐病菌Fusarium spp.侵染的代谢组分析及抗病标志物指认”(201801D121235);山西省科技重大专项“山西道地黄芪种质资源与规范化生产技术研究”(201603D3111001);山西省科技攻关项目中药现代化关键技术研究振东专项“黄芪根腐病专用微生物农药创制物质基础的筛选和评价”(2014ZD0501-2);山西省“1331工程”重点实验室——功能分子化学(晋教科[2017]14号)

Effects of Root Rot Pathogens Infection on Key Enzymes in Phenylpropanoid Pathway of Astragalus membranaceus var. mongholicus

Zhao Xuejiao¹^,²(), Qin Xuemei¹, Wang Mengliang³, Gao Fen³()

¹Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006
²College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006
³Institute of Applied Chemistry, Shanxi University, Taiyuan 030006

Received:2019-07-02 Revised:2019-11-05 Online:2020-09-05 Published:2020-08-18
Contact: Gao Fen

摘要/Abstract

摘要：

以蒙古黄芪-根腐病菌（Fusarium solani和F. acuminatum）为互作体系,测定苯丙烷途径关键酶苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟化酶(C4H)及4-香豆酸-辅酶A连接酶(4CL)活性的变化,以期获得黄芪抗根腐病菌侵染机制的信息,为抗病育种工作提供参考。试验采用幼苗浸根法接种病菌,分光光度法测定3种酶的动态变化。结果表明,F. solani侵染黄芪后,PAL和C4H活性与对照相比明显升高,并在7、21天出现2个酶活高峰;4CL活性则随着时间的延长持续上升,7、21天时也显著高于对照。F. acuminatum侵染黄芪后,PAL、C4H和4CL的活性均在21天显著高于对照。可见2种病原菌在侵染黄芪过程中,苯丙烷途径的关键酶被不同程度地激活,但2种病菌激活各酶的时间和规律有所差异,表明黄芪对这2种病菌的抗性机制明显不同。

关键词: 蒙古黄芪, 根腐病菌, 苯丙烷途径, 关键酶, 活性变化

Abstract:

In this study, the activity changes of key enzymes in phenylpropanoid pathway including phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:coenzyme A ligase (4CL) were determined to obtain information on the mechanism of Astragalus membranaceus against root rot pathogens, so as to provide reference for disease resistance breeding. Regarding A. membranaceus var. mongholicus and root rot pathogens as the interaction system, the dynamic activity changes of the three enzymes were measured by spectrophotometry methods with the A. membranaceus being inoculated with Fusarium solani and F. acuminatum respectively using the root-dipping method. The results showed that both PAL and C4H in Astragalus infected by F. solani had a significantly higher activity than their counterparts in the control, and both had enzyme activity peaks at 7 d and 21 d, respectively; the activity of 4CL steadily increased with the time and was also significantly higher than that of the control at 7 d and 21 d. In the case of Astragalus infected by F. acuminatum, the activities of all three enzymes in treatment were significantly higher than that in the control at 21 d. It can be seen that during the infection of both pathogens, the key enzymes in phenylpropanoid pathway are activated to some extents, but differ in the time and pattern of being activated, which indicates that A. membranaceus has obvious different disease-resistant mechanisms against the infection of F. solani and F. acuminatum.

Key words: Astragalus membranaceus var. mongholicus, root rot pathogens, phenylpropanoid pathway, key enzymes, activity change

中图分类号:

S432.4+4

赵雪姣, 秦雪梅, 王梦亮, 高芬. 根腐病菌侵染对黄芪苯丙烷途径关键酶活性的影响[J]. 中国农学通报, 2020, 36(25): 115-120.

Zhao Xuejiao, Qin Xuemei, Wang Mengliang, Gao Fen. Effects of Root Rot Pathogens Infection on Key Enzymes in Phenylpropanoid Pathway of Astragalus membranaceus var. mongholicus[J]. Chinese Agricultural Science Bulletin, 2020, 36(25): 115-120.

图/表 7

图1. F. solani和F. acuminatum 接种黄芪后发病情况

图2. F. solani侵染黄芪后PAL活性的变化不同字母表示对照组和接菌组在同一时期差异显著(P<0.05),下同

图3. F. acuminatum侵染黄芪后PAL活性的变化

图4. F. solani侵染黄芪后C4H活性的变化

图5. F. acuminatum侵染黄芪后C4H活性的变化

图6. F. solani侵染后4CL活性的变化

图7. F. acuminatum侵染后4CL活性的变化

参考文献 25

[1]	高芬, 任小霞, 王梦亮, 等. 中草药根腐病及其微生物防治研究进展[J]. 中国中药杂志, 2015,40(21):4122-4126.
[2]	高芬, 赵晓霞, 秦雪梅, 等. 山西省蒙古黄芪根腐病优势致病菌群分析[J].植物保护学报, 2018(4).
[3]	高微微, 张西梅, 田给林, 等. 探索中前行-中药材病害研究概况与思考[J]. 植物保护, 2016,42(5):15-23.
[4]	Arbona V, Aurelio Gómez-Cadenas. Metabolomics of Disease Resistance in Crops[J]. Current issues in molecular biology, 2015,19:13-30. URL pmid: 26364233
[5]	杜欣谊. 苯丙氨酸解氨酶的研究进展[J]. 现代化农业, 2016(7):24-26.
[6]	Luo Y, Bing-Zhi Li, Liu D, et al. Engineered biosynjournal of natural products in heterologous hosts[J]. Chemical Society Reviews, 2015,46(37):5265-5290.
[7]	Kostyn K, Czemplik M, Kulma A, et al. Genes of phenylpropanoid pathway are activated in early response to Fusarium attack in flax plants[J]. Plant Science An International Journal of Experimental Plant Biology, 2012,190:0-115.
[8]	Mandal S, Mallick N, Mitra A. Salicylic acid-induced resistance to Fusarium oxysporum f. sp. lycopersici in tomato[J]. Plant Physiology & Biochemistry, 2009,47(7):642-649. doi: 10.1016/j.plaphy.2009.03.001 URL pmid: 19329332
[9]	Guiyun Lü, Guo S, Zhang H, et al. Transcriptional profiling of watermelon during its incompatible interaction with Fusarium oxysporum f. sp. niveum[J]. European Journal of Plant Pathology, 2011,131(4):585-601. doi: 10.1007/s10658-011-9833-z URL
[10]	Madadkhah E, Lotfi M, Nabipour A, et al. Enzymatic activities in roots of melon genotypes infected with Fusarium oxysporum f. sp. melonis race 1[J]. Scientia Horticulturae, 2012,135:1-176. doi: 10.1016/j.scienta.2011.12.005 URL
[11]	Ma L, He J, Liu H, et al. The phenylpropanoid pathway affects apple fruit resistance to Botrytis cinerea[J]. Journal of Phytopathology, 2017,166(3):206-215. doi: 10.1111/jph.2018.166.issue-3 URL
[12]	李捷, 冯丽丹, 杨成德, 等. 接种尖镰孢菌对枸杞苯丙烷代谢关键酶及产物的影响[J]. 草业学报, 2016,25(5):87-94. doi: 10.11686/cyxb2016024 URL
[13]	包改红, 毕阳, 李永才, 等. 硫色镰刀菌(Fusarium sulphureum)接种对抗病/易感品种马铃薯块茎苯丙烷代谢的影响比较[J]. 食品科学, 2015,36(6):251-256. doi: 10.7506/spkx1002-6630-201506048 URL
[14]	赵庆芳, 陈玉萍, 李巧峡, 等. 黄芪感染根腐病菌后抗氧化系统的变化规律研究[J]. 西北师范大学学报:自然科学版, 2013,49(2):71-74.
[15]	岳换弟, 秦雪梅, 王梦亮, 等. 活体外黄芪根腐病菌细胞壁降解酶产生能力比较[J]. 湖北农业科学, 2017(22):122-127.
[16]	岳换弟. 基于酶学响应及代谢组变化的根腐病菌Fusarium spp.-黄芪互作研究[D]. 太原:山西大学, 2018.
[17]	何利, 唐翠明, 戴凡炜. 防御酶与植物抗青枯病关系研究进展[J]. 广东农业科学, 2013,40(12):101-103.
[18]	Zabala G, Zou J, Tuteja J, et al. Transcriptome changes in the phenylpropanoid pathway of Glycine maxin response to Pseudomonas syringae infection[J]. Bmc Plant Biology, 2006,6(1):26. doi: 10.1186/1471-2229-6-26 URL
[19]	Sun H, Li Y, Feng S, et al. Analysis of five rice 4-coumarate: coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynjournal in rice[J]. Biochemical and Biophysical Research Communications, 2013,430(3):1151-1156. doi: 10.1016/j.bbrc.2012.12.019 URL pmid: 23246835
[20]	王辉. 青枯菌接种后花生抗感2个品种中几种防御性酶活性的变化[D]. 武汉:中南民族大学, 2011.
[21]	裴冬丽, 张红岩, 张贺, 等. 干旱胁迫对番茄幼苗叶片SOD、POD和PAL活性的影响[J]. 吉林农业科学, 2015,40(4):83-86.
[22]	陈雷, 常丽, 曹福亮, 等. 银杏叶黄酮类化合物含量及相关酶活性对温度和干旱胁迫的响应[J]. 西北植物学报, 2013,33(4):755-762.
[23]	吴洋, 房敏峰, 岳明, 等. UV-B辐射对药用植物次生代谢的影响研究进展[J]. 中国中药杂志, 2012,37(15):2247-2251.
[24]	张必弦, 胡小梅, 朱延明, 等. 野生大豆苯丙氨酸解氨酶(PAL)在不同诱导条件下变化规律[J]. 大豆科学, 2011,30(4):703-705.
[25]	吴琼, 方吴云, 王文杰, 等. 机械损伤诱导植物苯丙氨酸解氨酶活性研究进展[J]. 现代农业科技, 2016(1):157-158.

根腐病菌侵染对黄芪苯丙烷途径关键酶活性的影响

Effects of Root Rot Pathogens Infection on Key Enzymes in Phenylpropanoid Pathway of Astragalus membranaceus var. mongholicus

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 25

相关文章 12

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	张婷婷, 马贵龙, 谢晓宝, 高新馨, 蔡奇. 贝莱斯芽孢杆菌SX-45提取物对人参根腐病菌抑菌机理研究[J]. 中国农学通报, 2022, 38(33): 124-131.
[2]	丁昊, 刘文娟, 孙健, 刘磊, 平文祥, 葛菁萍. 高产2,3-丁二醇的潜在酿酒酵母菌株筛选[J]. 中国农学通报, 2020, 36(24): 107-115.
[3]	王晓飞, 魏国江, 关向军, 刘淑霞, 肖宇, 吴耀坤, 徐海军. 密度与环境因子对蒙古黄芪育苗影响研究[J]. 中国农学通报, 2020, 36(15): 43-47.
[4]	唐宏亮,石瑛,田洵,王金明. 马铃薯淀粉合成关键酶与块茎淀粉积累的关系[J]. 中国农学通报, 2015, 31(27): 88-93.
[5]	吕婧刘贯山晁江涛孙玉合. 影响烟草香气物质合成代谢途径关键酶基因的研究进展[J]. 中国农学通报, 2014, 30(4): 49-57.
[6]	席旭东. 纳米硒植物营养剂对蒙古黄芪产量及品质的影响[J]. 中国农学通报, 2014, 30(25): 262-266.
[7]	宋光桃周国英. 土壤拮抗细菌的分离与筛选[J]. 中国农学通报, 2012, 28(27): 99-103.
[8]	郑域茹谢宝贵陈柄智. 洛巴伊口蘑生长过程中几种胞外酶活性变化[J]. 中国农学通报, 2011, 27(25): 261-265.
[9]	夏斌郭涛王慧刘永柱张建国陈志强. 水稻淀粉合成关键酶的研究进展[J]. 中国农学通报, 2009, 25(22): 0-0.
[10]	张晓燕,俞杏珍,符云鹏,黄若平,孟菲. 不同基因型香料烟叶片发育过程中碳氮代谢关键酶活性及代谢产物变化[J]. 中国农学通报, 2009, 25(12): 112-116.
[11]	徐一兰,官春云,,谭太龙. 油菜种子油分形成的生理生化基础研究进展[J]. 中国农学通报, 2006, 22(4): 255-255.
[12]	张淑珍,徐鹏飞,武小霞,赵孝武,张大勇,姜振峰,李文滨. 大豆疫霉根腐病菌毒素的提取及生物活性测定[J]. 中国农学通报, 2005, 21(3): 252-252.