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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (25): 121-128.doi: 号：10.11924/j.issn.1000-6850.casb20190900639

所属专题： 园艺

• 植物保护·农药 • 上一篇    下一篇

南瓜侵染白粉菌后的活性氧爆发观察

秦宏坤1,2(), 李帅1,2, 马冬1,2, 郭卫丽1,2()   

  1. 1河南科技学院园艺园林学院,河南新乡 453003
    2河南省园艺植物资源利用与种质创新工程研究中心,河南新乡 453003
  • 收稿日期:2019-09-14 修回日期:2019-11-17 出版日期:2020-09-05 发布日期:2020-08-18
  • 通讯作者: 郭卫丽
  • 作者简介:秦宏坤,男,1996年出生,河南郑州人,本科,研究方向：瓜类白粉病的抗性机制。通信地址：453003 河南省新乡市红旗区河南科技学院,E-mail： 1107818007@qq.com。
  • 基金资助:
    国家自然科学基金青年基金项目“南瓜抗白粉病基因的分离及功能鉴定”(31401876);河南省博士后高层人才引进项目“南瓜基因CmSGT1在白粉病抗性方面的功能”(103020218003/008);大学生“百农英才”创新项目“南瓜幼苗期侵染白粉病后的超敏反应”(BNYC2017-2-35);河南省大学生创新训练项目(2019CX086);河南省重点研发与推广专项(182102110049)

Active Oxygen Outbreaks of Pumpkin Infected with Powdery Mildew

Qin Hongkun1,2(), Li Shuai1,2, Ma Dong1,2, Guo Weili1,2()   

  1. 1School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang Henan 453003
    2Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang Henan 453003
  • Received:2019-09-14 Revised:2019-11-17 Online:2020-09-05 Published:2020-08-18
  • Contact: Guo Weili

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摘要：

为探究不同抗性南瓜材料在感染白粉病后活性氧积累水平的变化情况,对中国南瓜自交系112-2和‘九江轿顶’侵染白粉菌后的活性氧爆发情况进行观察。结果表明：在幼苗期,自交系112-2和‘九江轿顶’0 h接种白粉菌孢子,72 h检测到H2O2开始积累,96~144 h H2O2积累量逐步增加。自交系112-2和‘九江轿顶’0 h接种白粉菌孢子,24 h开始检测到O2-,48~144 h O2-积累量逐步增加。在结果期,2种材料在0 h接种白粉菌孢子,48 h自交系112-2开始检测出H2O2,72~144 h H2O2积累量逐渐增多;而‘九江轿顶’72 h开始检测出H2O2,96~144 h H2O2积累量逐渐增多。自交系112-2和‘九江轿顶’0 h接种白粉菌孢子,48 h开始检测到O2-,72~144 h O2-积累量逐步增加。中国南瓜自交系112-2比‘九江轿顶’产生H2O2和O2-的过程迅速,且积累量差异在第144 h达到最大。抗性材料接种白粉菌后H2O2和O2-的积累发生较快,较多于感病材料。

关键词: 南瓜, 白粉菌, ROS, H2O2, O2-

Abstract:

The active oxygen outbreaks of pumpkin inbred line 112-2 and ‘Jiujiangjiaoding’ infected with powdery mildew were observed to explore the correlation of ROS with the resistance of pumpkin to powdery mildew. DAB-dyed spots represented H2O2 accumulation and NBT-dyed spots represented O2- accumulation. The results showed that at the seedling stage, the inbred line 112-2 and ‘Jiujiangjiaoding’ were inoculated with powdery spores at 0 h, and a small amount of DAB-dyed spots were observed at 72 h, and the DAB-dyed spots expanded and deepened from 96 h to 144 h. The inbred line 112-2 and ‘Jiujiangjiaoding’ were inoculated with powdery spores at 0 h, NBT-dyed spots began to appear at 24 h, the NBT-dyed spots continued to expand and deepened from 48 h to 144 h. In the fruiting period, the two materials were inoculated with powdery mildew spores at 0 h, DAB-dyed spots were observed in inbred line 112-2 at 48 h, and the spots enlarged and deepened from 72 h to 144 h; DAB-dyed spots were observed at 72 h from the ‘Jiujiangjiaoding’, and the spots expanded and deepened from 96 h to 144 h. The inbred line 112-2 and ‘Jiujiangjiaoding’ were inoculated with powdery spores at 0 h; NBT-dyed spots began to appear at 48 h, the NBT-dyed spots expand and deepened from 72 h to 144 h. The inbred line 112-2 produced H2O2 and O2- faster than the ‘Jiujiangjiaoding’ and the difference in accumulation amount reached the maximum at 144 h. In conclusion, the accumulation of H2O2 and O2- in the resistant material inoculated with powdery mildew is faster and more than the susceptible material.

Key words: pumpkin, powdery mildew, ROS, H2O2, O2-

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