H2O2溶液对 2种红葡萄离体叶中主要茋化物含量的影响

doi:10.11924/j.issn.1000-6850.2012-2660

摘要/Abstract

摘要： 比较H₂O₂诱导后美国红提（‘红地球’）和紫香无核（‘新农4号’）离体葡萄叶片中的白藜芦醇(Res)、白藜芦醇苷(PD)、紫檀茋(PS)、ε-二聚体葡萄素(ε-VF) 4种茋化物的含量，为利用葡萄生产废弃叶片的综合利用提供依据。采用H₂O₂溶液对红提和紫香无核离体葡萄叶片进行诱导，采用高效液相色谱法分析4种茋化物的含量变化。H₂O₂诱导后的两种离体叶片4种茋化物的积累呈现显著的量时依赖性；2.5% H₂O₂处理48 h后叶片4种茋化物含量可提高6倍以上，以红提的含量相对较高，总茋化物鲜重达到138.82μg/g。用H₂O₂诱导后的离体葡萄叶中4种茋化物含量比诱导前有了较为明显的增加。

关键词: 预测, 预测

Abstract: The aims were to compare the contents of resveratrol(Res), piceide(PD), pterostilbene (PS),ε-viniferin (ε-VF) in the grape leaves of Hongti (‘Vitis vinifera cv. Red globe’) and Zixiangwuhe (‘Vitis vinifera cv. NO.4 Xin nong’) treated with H₂O₂, and provide the basis for the comprehensive utilization of waste grape leaves. In present study, the detached grape leaves Hongti and Zixiangwuhe were treated with H₂O₂ solution to investigate the changes of stilbenes contents by HPLC methods. The results showed those stilbenes accumulated significantly in both dose-dependent and time-dependent manners in H₂O₂-treated leaves. 4 kinds of stilbenes increased 6 times higher than untreated, with 2.5% H₂O₂ for 48 hours treatment. Stilbenes contents of Hongti grape leaves was relatively high, the maximum contents of the total stilbenes compounds was 138.82μg/g in treated leaves. H₂O₂-treated detached grape leaves showed notably increase on stilbenes contents than blank.

田春芳张波刘景磊李荣飞王晓琴陈韩英. H₂O₂溶液对 2种红葡萄离体叶中主要茋化物含量的影响[J]. 中国农学通报, 2013, 29(6): 85-90.

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H₂O₂溶液对 2种红葡萄离体叶中主要茋化物含量的影响

The Impact of H₂O₂ Treatment on Stilbene Contents in the Detached Leaves of Two Cultivars Red Grapevine

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