不同破眠处理对油桃休眠芽H2O2含量及 相关酶活性的影响

doi:10.11924/j.issn.1000-6850.2014-1331

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 128-132.doi: 10.11924/j.issn.1000-6850.2014-1331

所属专题：生物技术

不同破眠处理对油桃休眠芽H₂O₂含量及相关酶活性的影响

谭钺,王茂生,吕勐,崔海金

山东省果树研究所,山东省莒南县望海楼国有林场,青岛市动物园,山东省果树研究所

收稿日期:2014-05-08 修回日期:2014-05-08 接受日期:2014-08-06 出版日期:2014-10-15 发布日期:2014-10-15
通讯作者: 谭钺
基金资助:
山东省现代农业产业技术体系水果创新团队专项基金(SDAIT-03-022-04)

Effects of Different Dormancy-breaking Treatments on H₂O₂ Content and Activity of Related Enzymes of Dormant Nectarine Buds

Received:2014-05-08 Revised:2014-05-08 Accepted:2014-08-06 Online:2014-10-15 Published:2014-10-15

摘要/Abstract

摘要： 为进一步探讨H₂O₂代谢与果树芽自然休眠解除的关系，检测了人工破眠过程中‘曙光’油桃芽内H2O2含量和过氧化氢酶(CAT)、过氧化物酶(POD)活性的影响。选用的人工破眠处理分别为50℃高温(HT)处理、单氰胺(HC)处理和TDZ(TDZ)处理。结果表明，50℃高温和单氰胺效果相似，都能显著打破芽深休眠，并且抑制芽内CAT活性，引起H₂O₂含量增加，但2 个处理对POD活性的影响都不大。TDZ打破深休眠的效果较差，对芽内H2O2含量、CAT 活性、POD活性均未表现出显著影响。CAT 活性抑制和H₂O₂积累可能是50℃高温和单氰胺打破自然休眠作用机制的重要组成部分。

关键词: 品质, 品质

Abstract: In order to further explore relationships between bud dormancy release and H₂O₂ metabolism, changes of H₂O₂ level, CAT activity and POD activity of‘Shuguang’nectarine buds during artificial dormancybreaking process were studied. High temperature of 50℃(HT), hydrogen cyanamide (HC) and TDZ (TDZ) were selected as dormancy-breaking treatments. Both HT and HC treatments significantly breaking bud dormancy, inhibited CAT activity and induced H₂O₂ accumulation, but showed little effects on POD activity. TDZ showed poor dormancy-breaking efficiency and no significant effects on H₂O₂ content, CAT activity and POD activity. Inducing H₂O₂ accumulation through CAT inhibition might be an important part of dormancy- breaking mechanism induced by HT and HC.

谭钺,王茂生,吕勐,崔海金. 不同破眠处理对油桃休眠芽H₂O₂含量及相关酶活性的影响[J]. 中国农学通报, 2014, 30(28): 128-132.

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不同破眠处理对油桃休眠芽H₂O₂含量及相关酶活性的影响

Effects of Different Dormancy-breaking Treatments on H₂O₂ Content and Activity of Related Enzymes of Dormant Nectarine Buds

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