外源激素与基质对黄果厚壳桂扦插生根的影响

doi:10.11924/j.issn.1000-6850.casb2020-0282

摘要/Abstract

摘要：

为提高黄果厚壳桂扦插生根率和苗木成活率,探讨该树种的扦插生根特性,分析外源激素和基质对扦插的影响,以期为黄果厚壳桂的苗木繁育和树种扩繁提供科学参考。以黄果厚壳桂为研究对象,采用L₉(3⁴)正交设计开展扦插试验,通过方差分析、相关分析和主成分分析3种方法阐明外源激素和基质对该树种扦插生根的影响。结果表明,使用黄心土做扦插基质,黄果厚壳桂的扦插生根率最高,达70.0%,显著大于河沙与泥炭土+椰糠。在不同外源激素处理中,IAA处理的枝条扦插生根率和抽梢率最高,分别为66.7%和52.2%。激素浓度为250 mg/L时,扦插生根率最高,并随激素浓度升高而降低,但基部愈伤率效果相反。不同浸泡时间处理对黄果厚壳桂的扦插生根率和基部愈伤率的影响不同,其中30 min处理的生根率最高,而10 min处理的基部愈伤率最高。综合3种统计分析方法的研究结果,以黄心土为扦插基质,使用浓度为250 mg/L的IBA生根液浸泡插穗基部10 min,黄果厚壳桂扦插生根和抽梢效果最好,生根率和抽梢率分别是76.7%和70.0%。

关键词: 黄果厚壳桂, 正交试验, 外源激素, 基质, 扦插

Abstract:

To improve the rooting rate and survival rate of the seedlings, the rooting characteristics of Cryptocarya concinna were discussed, and the influence of exogenous hormones and matrix factors on the cutting was analyzed, so as to provide scientific reference for the seedling breeding and tree propagation. The effects of exogenous hormones and substrate on cutting rooting were studied by means of variance analysis, correlation analysis and principal component analysis, using L₉(3 ⁴) orthogonal experiment design and taking the species of C. concinna as research objects. The results showed that: taking yellow soil as the substrate, the rooting rate of C. concinna was the highest, up to 70.0%, which was significantly higher than that of river sand, peat soil and coconut. IAA treatment had the highest rooting rate and sprouting rate, up to 66.7% and 52.2%, among different hormone treatments. The rooting rate of cuttings was the highest by the hormone concentration of 250 mg/L and decreased with the increase of hormone concentration, but the basal callus rate was the opposite. The influence of different soaking time on the rooting rate and the basal callus rate of C. concinna was different, with the highest rooting rate in the 30 min treatment and the highest basal callus rate in the 10 min treatment. Based on the results of three statistical analysis methods, it is found that the effect of rooting and sprouting on cutting of C. concinna is the best with the rooting rate of 76.7% and the sprouting rate of 70.0% when the yellow soil is used as the cutting substrate, and IBA rooting solution with a concentration of 250 mg/L is used to soak the base of cuttings for 10 min.

Key words: Cryptocarya concinna, orthogonal experiment, exogenous hormones, substrate, cutting

中图分类号:

梁小春, 黄松殿, 姜仪民, 赵春梅. 外源激素与基质对黄果厚壳桂扦插生根的影响[J]. 中国农学通报, 2021, 37(10): 24-29.

Liang Xiaochun, Huang Songdian, Jiang Yimin, Zhao Chunmei. Effects of Exogenous Hormones and Substrate on Cutting Rooting of Cryptocarya concinna[J]. Chinese Agricultural Science Bulletin, 2021, 37(10): 24-29.

图/表 7

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编号	生根率/%	基部愈伤率/%	抽梢率/%	抽梢长度/cm	一级根数/条	一级根长/cm	二级根数/条	单株抽梢数/条
处理1	36.7e	46.7a	33.3b	2.111b	2.110	6.320	6.890	0.890
处理2	53.3cd	30.0b	36.7b	3.160ab	1.600	7.287	8.733	1.733
处理3	60.0bc	13.3c	63.3a	4.407ab	1.767	6.717	7.177	1.923
处理4	60.0bc	10.0c	56.7a	4.650ab	2.387	11.700	10.750	1.390
处理5	76.7a	10.0c	70.0a	6.071a	2.187	11.453	11.100	1.863
处理6	73.3ab	13.3c	66.7a	6.685a	2.487	9.953	8.237	1.400
处理7	70.0ab	13.3c	36.7b	1.401b	2.137	9.710	9.513	0.453
处理8	43.3de	16.7c	33.3b	1.108b	2.017	11.683	8.167	0.650
处理9	66.7ab	20.0bc	26.7b	1.930b	2.570	8.493	11.130	0.560

编号	生根率/%	基部愈伤率/%	抽梢率/%	抽梢长度/cm	一级根数/条	一级根长/cm	二级根数/条	单株抽梢数/条
处理1	36.7e	46.7a	33.3b	2.111b	2.110	6.320	6.890	0.890
处理2	53.3cd	30.0b	36.7b	3.160ab	1.600	7.287	8.733	1.733
处理3	60.0bc	13.3c	63.3a	4.407ab	1.767	6.717	7.177	1.923
处理4	60.0bc	10.0c	56.7a	4.650ab	2.387	11.700	10.750	1.390
处理5	76.7a	10.0c	70.0a	6.071a	2.187	11.453	11.100	1.863
处理6	73.3ab	13.3c	66.7a	6.685a	2.487	9.953	8.237	1.400
处理7	70.0ab	13.3c	36.7b	1.401b	2.137	9.710	9.513	0.453
处理8	43.3de	16.7c	33.3b	1.108b	2.017	11.683	8.167	0.650
处理9	66.7ab	20.0bc	26.7b	1.930b	2.570	8.493	11.130	0.560

基质	生根率/%	基部愈伤率/%	抽梢率/%	一级根长/cm	抽梢长度/cm	单株抽梢数/条	一级根数/条	二级根数/条
河沙	50.0c	30.0a	44.4b	6.886b	3.226b	1.515a	1.826	7.600
黄心土	70.0a	11.1b	64.4a	11.034a	5.802a	1.551a	2.354	10.030
泥炭土+椰糠	60.0b	16.7b	32.2c	9.962a	1.480b	0.555b	2.240	9.715

基质	生根率/%	基部愈伤率/%	抽梢率/%	一级根长/cm	抽梢长度/cm	单株抽梢数/条	一级根数/条	二级根数/条
河沙	50.0c	30.0a	44.4b	6.886b	3.226b	1.515a	1.826	7.600
黄心土	70.0a	11.1b	64.4a	11.034a	5.802a	1.551a	2.354	10.030
泥炭土+椰糠	60.0b	16.7b	32.2c	9.962a	1.480b	0.555b	2.240	9.715

处理	水平	生根率/%	基部愈伤率/%	抽梢率/%	一级根长/cm	一级根数/条	二级根数/条	抽梢长度/cm	单株抽梢数/条
外源激素种类	NAA	55.6b	23.3	42.2b	9.244	2.213	9.051	2.721	0.911
	IBA	57.8b	18.9	46.7ab	10.140	1.934	9.334	3.447	1.416
	IAA	66.7a	15.6	52.2a	8.498	2.273	8.960	4.340	1.294
外源激素浓度	1000 mg/L	51.1c	25.6a	44.4b	9.319	2.205	7.765	3.301	0.979
	500 mg/L	60.0b	20.0a	40.0b	9.160	2.185	10.316	3.247	1.227
	250 mg/L	68.9a	12.2b	56.7a	9.403	2.030	9.264	3.960	1.414
外源激素浸泡时间	10 min	60.0ab	25.6a	43.3	8.756	2.287	9.818	3.371	1.105
	30 min	65.6a	18.9ab	46.7	8.983	2.075	8.828	3.748	1.196
	60 min	54.4b	13.3b	51.1	10.144	2.057	8.698	3.388	1.320