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中国农学通报 ›› 2023, Vol. 39 ›› Issue (5): 39-48.doi: 10.11924/j.issn.1000-6850.casb2022-0773

• 林学·园艺·园林 • 上一篇    下一篇

杨树杂交F1代扦插无性系苗期生长节律及遗传变异分析

闫彩霞1(), 苗婷婷1, 刘俊龙1, 吴中能1(), 苏晓华2, 丁昌俊2, 曹志华1, 孙慧1   

  1. 1 安徽省林业科学研究院,合肥 230088
    2 中国林业科学研究院林业研究所,北京100091
  • 收稿日期:2022-09-13 修回日期:2022-11-15 出版日期:2023-02-15 发布日期:2023-02-06
  • 作者简介:

    闫彩霞,女,1989年出生,河南濮阳人,研实员,硕士,研究方向:森林培育。通信地址:230088 安徽省合肥市蜀山区黄山路618-1号 安徽省林业科学研究院,Tel:0551-62631176,E-mail:

  • 基金资助:
    科技部国家重点研发计划项目“杨树大径级工业资源材精准高效培育技术研究”(2021YFD2201200); 安徽省科技厅“2022年安徽现代林业发展及科研能力提升项目”

The Growth Rhythm and Genetic Variation of the F1 Hybrid Cuttings of Populus deltoids

YAN Caixia1(), MIAO Tingting1, LIU Junlong1, WU Zhongneng1(), SU Xiaohua2, DING Changjun2, CAO Zhihua1, SUN Hui1   

  1. 1 Anhui Academy of Forestry, Hefei 230088
    2 Forestry Research Institute, Chinese Academy of Forestry, Beijing 100091
  • Received:2022-09-13 Revised:2022-11-15 Online:2023-02-15 Published:2023-02-06

摘要:

研究杨树杂交F1代扦插无性系苗期生长节律及遗传变异,旨在为杨树遗传改良和优质种质资源挖掘提供依据。以‘I-69’杨为母本,‘I-63’杨为父本,将通过控制授粉杂交试验得到的30个F1代及2个杂交亲本作为研究对象,开展大田扦插试验,分析参试材料在苗期苗高和地径上的生长进程及遗传变异。结果显示1年生杨树F1代扦插无性系苗高和地径生长符合“S”型曲线,并能用Logistic方程很好的拟合其动态生长节律;不同无性系的苗高和地径均达极显著水平(P<0.01),超亲优势显著,偏母本效应;2个生长性状的重复力分别是0.88、0.72,变幅分别为125.11%、108.94%,表型变异系数分别为23.67%、19.97%,遗传变异系数分别为40.37%、25.98%;2个生长性状的重复力、表型变异系数、遗传变异系数均随月龄先增后减,遗传方差、环境方差和表型方差基本上随月龄增加而增大,遗传方差效应对表型方差贡献率逐月增大。以苗高和地径为评价指标,结合聚类分析筛选出5个生长性状表现好的无性系,苗高和地径的平均值分别为3.10m和19.92 mm,遗传增益分别在12.48%~41.96%和10.52%~21.83%之间,可作为开展林木营林生产的材料,同时为杨树进一步遗传改良和品种更新提供参考。

关键词: 杨树, 杂交F1代, Logistic生长方程, 遗传变异

Abstract:

The growth rhythm and genetic variation of the cutting clones from the F1 hybrid of poplar were investigated to provide a basis for poplar genetic improvement and high-quality germplasm resources mining. Using 30 F1 hybrids obtained by the controlled pollination hybridization experiment with poplar ‘I-69’ as the female parent and poplar ‘I-63’ as male parent and the two parents as the research objects, we carried out a field cutting experiment to analyze the growth process and genetic variation of the seedling height and ground diameter of the experimental materials at seedling stage. Results showed that these two growth traits of the 1-year-old cuttings from the F1 hybrid of poplar were in line with the ‘S’ curve, and the Logistic growth equation could fit the dynamic growth rhythm well. The seedling height and ground diameter of different clones were significantly different (P<0.01), indicating obvious super-parental advantage and significant maternal parent effect. The repeatability, variation range, phenotypic variation coefficient, and the genetic variation coefficient of the two growth traits were 0.88 and 0.72, 125.11% and 108.94%, 23.67% and 19.97%, and 40.37% and 25.98%, respectively. The repeatability, phenotypic variation coefficient, and genetic variation coefficient of the two growth traits increased first and then decreased, while the genetic variance, environmental variance and phenotypic variance basically increased with growing months, and the contribution rate of genetic variance effect to phenotypic variance increased month by month. Based on the cluster analysis using seedling height and ground diameter as evaluation indexes, five clones with good growth performance traits were selected. The average seedling height and ground diameter of the five selected clones were 3.10 m and 19.92 mm, and the genetic gains were 12.48%-41.96% and 10.52%-21.83%, respectively. The five selected clones could be used as materials for forest production and poplar variety improvement.

Key words: Populus deltoids, F1 hybrid, Logistic growth equation, genetic variation