外源激素配比对铁皮石斛茎段快繁的影响

doi:10.11924/j.issn.1000-6850.casb2024-0631

摘要/Abstract

摘要： 铁皮石斛无性快繁各阶段对植物激素的需求不同。为探究外源激素配比与铁皮石斛组培苗生长之间的关系，以浙江省天目山的铁皮石斛带节茎段为外植体，进行铁皮石斛丛生芽诱导、增殖和生根试验，比较各试验阶段激素配比对铁皮石斛生长的影响，从而筛选出适合铁皮石斛茎段快繁的最佳激素条件，建立铁皮石斛茎段无性快繁体系。结果表明，丛生芽诱导最佳激素条件为1.0 mg/L NAA+2.0 mg/L 6-BA，发芽率97.8%，芽健壮；丛生芽增殖的最佳激素条件为0.5 mg/L NAA+2.0 mg/L 6-BA，增殖率86.9%，植株长势良好；丛生芽生根阶段的最佳激素条件为1.0 mg/L NAA+1.0 mg/L IBA，生根率为83.4%，根系健壮发达且无黄化现象。研究证实，激素配比与培养基盐浓度协同调控铁皮石斛形态发生，其中7~8叶期为生理响应关键节点。本研究筛选出的激素组合可为兰科药用植物规模化组培提供理论依据与技术参考。

关键词: 铁皮石斛, 茎段快繁, 外源激素, 诱导, 丛生芽, 增殖, 生根

Abstract:

The demand for plant hormones varies in different stages of asexual rapid propagation of Dendrobium officinale. The paper aims to further explore the relationship between exogenous hormone ratio and tissue culture seedling growth. The segmented stem segments of D. officinale from Tianmushan of Zhejiang were used as explants to conduct experiments on induction, proliferation, and rooting of clustered buds of D. officinale. The effects of different hormone ratios on the growth of D. officinale at different stages were compared, and the optimal hormone conditions for rapid propagation of D. officinale stem segments were selected to establish an asexual rapid propagation system of D. officinale stem segments. The results showed that the optimal hormone conditions for inducing clustered buds were 1.0 mg/L NAA+ 2.0 mg/L 6-BA, with a germination rate of 97.8% and robust buds. The optimal hormone conditions for the proliferation of clustered buds of D. officinale were 0.5 mg/L NAA+ 2.0 mg/L 6-BA, with a proliferation rate of 86.9% and good growth. The optimal hormone conditions for the rooting of clustered buds of D. officinale were 1.0 mg/L NAA+ 1.0 mg/L IBA, with a rooting rate of 83.4% and a robust and developed root system. The study confirmed that hormone ratio and medium salt concentration synergistically regulated the morphogenesis of D. officinale, and the 7-8 leaf stage was the key node of physiological response. The hormone combinations screened in this study can provide theoretical basis and technical reference for large-scale tissue culture of orchid medicinal plants.

Key words: Dendrobium officinale, rapid propagation of stem segments, exogenous hormones, induction, clustered buds, proliferation, rooting

佘娜, 张焊, 冯月, 陈功轩, 李景蕻. 外源激素配比对铁皮石斛茎段快繁的影响[J]. 中国农学通报, 2025, 41(19): 42-48.

SHE Na, ZHANG Han, FENG Yue, CHEN Gongxuan, LI Jinghong. Effects of Exogenous Hormones Ratio on Rapid Propagation of Stem Segments of Dendrobium officinale[J]. Chinese Agricultural Science Bulletin, 2025, 41(19): 42-48.

图/表 9

参考文献 28

[1]	汤久杨, 陈兰芬, 马喆. 北京地区铁皮石斛设施化栽培技术研究[J]. 北京农业职业学院学报, 2017(2):24-28.
[2]	罗仲春. 铁皮石斛原生态栽培技术[M]. 北京: 中国林业出版社, 2013:3.
[3]	朱波, 铁皮石斛人工杂交技术与优良家系苗期选择[D]. 杭州: 浙江农林大学, 2011.
[4]	王春, 郑勇平, 罗蔓, 等. 铁皮石斛试管苗快繁体系[J]. 浙江林学院学报, 2007, 24(3):372-376.
[5]	VELLUPILLAI M, SWARUP S, CHONG J H. Histological and protein chang during early stages of seed germination in the orchid, Dendrobium erumenatwn[J]. Journal of horticultural science, 1997, 72:941-948.
[6]	李景蕻, 张丽华, 张宇. 铁皮石斛不同授粉方式对蒴果发育及种子萌发的影响[J]. 基因组学与应用生物学, 2018, 37(1):413-417.
[7]	国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2010:265-266.
[8]	李景蕻, 张丽华, 钟静, 等. 不同菌根真菌对铁皮石斛穴盘苗生长及生根的影响[J]. 华北农学报, 2022,37:172-177.
[9]	陈兰芬, 汤久杨, 马喆. 铁皮石斛组织培养研究[J]. 北京农业职业学院学报, 2018(1):24-29.
[10]	李景蕻, 张丽华, 张宇. 中药材铁皮石斛组培苗不同培养基的筛选与优化[J]. 基因组学与应用生物学, 2018, 37(6):2551-2557.
[11]	常美花, 金亚征, 王莉. 铁皮石斛快繁技术体系研究[J]. 中草药, 2012, 43(7):1412-1417.
[12]	殷丽青, 孙翊, 周音, 等. 添加物和植物生长调节剂对铁皮石斛离体快速繁殖的影响[J]. 上海交通大学学报(农业科学版), 2014, 32(4):8-13.
[13]	付传明, 赵志国, 黄宁珍, 等. 铁皮石斛无菌播种产业化繁育技术研究[J]. 广西植物, 2012, 32(2):238-242.
[14]	李景蕻, 张丽华, 钟静. 金线莲和白及组培苗菌根真菌接种效应研究[J]. 中国农学通报, 2024, 40(24):122-128. doi: 10.11924/j.issn.1000-6850.casb2023-0506
[15]	朱根发, 叶秀斌, 陈明莉, 等. 培养基不同成分对墨兰根状茎分化成苗的影响[J]. 中南林学院学报, 2003, 23(5):42-44.
[16]	JOHNSON T R, KANE M E. Asymbiotic germination of ornamental vanda: In vitro germination and development of three hybrids[J]. Plant cell, tissue and organ culture, 2007, 91(3):251-261.
[17]	KAUTH P J, VENDRAME W A, KANE M E. In vitro seed culture and seedling development of Calopogon tuberosus[J]. Plant cell, tissue and organ culture, 2006, 85(1):91-102.
[18]	DIJK E, ECK N. Axenic in vitro nitrogen and phosphorus responses of some Dutch marsh orchids[J]. New phytologist, 2010, 131(3):353-359.
[19]	张金芳, 殷浩, 叶晶晶, 等. 培养基成分对组培桑苗叶片中1-脱氧野尻霉素含量的影响[J]. 蚕业科学, 2008, 34(3):400-404.
[20]	江金兰. 激素和有机质对铁皮石斛×重唇石斛F₁代增殖的影响[J]. 亚热带农业研究, 2022, 18(4):252-258.
[21]	杨平飞, 吴明开, 宋智琴, 等. 不同激素配比对铁皮石斛组培丛生芽增殖的影响[J]. 北方园艺, 2016(6):106-108.
[22]	戴忠华, 樊柳园, 朱华, 等. 植物激素及遮阳处理对铁皮石斛多糖含量的影响[J]. 分子植物育种, 2018, 16(23):7813-7820.
[23]	袁颖丹, 李志, 胡冬南, 等. 铁皮石斛组织培养研究进展[J]. 湖北农业科学, 2016(1):9-12.
[24]	曾铭. 铁皮石斛组织培养研究进展[J]. 民营科技, 2018(3):91-92.
[25]	陈苗, 崔世钢. 组培铁皮石斛LED智能照明系统设计[J]. 天津职业技术师范大学学报, 2021, 31(1):42-47.
[26]	吴瑶, 王再花, 李杰, 等. LED光质对2种石斛兰组培苗增殖、生根及生长的影响[J]. 中国农学通报, 2023, 39(13):64-69. doi: 10.11924/j.issn.1000-6850.casb2022-0435
[27]	杨欣盛, 王玉英, 赵一燃, 等. 不同多胺对铁皮石斛瓶内开花的影响[J]. 湖南农业科学, 2022(1):60-63.
[28]	戴含晶, 陈金鑫, 王宁, 等. 石榴组培苗无糖培养条件的初步研究[J]. 安徽科技学院学报, 2023, 37(6):35-40.

处理	基本培养基	NAA/(mg/L)	6-BA/(mg/L)
A1	MS	0.5	1.0
A2	MS	0.5	2.0
A3	MS	0.5	3.0
A4	MS	1.0	1.0
A5	MS	1.0	2.0
A6	MS	1.0	3.0
A7	1/2MS	0.5	1.0
A8	1/2MS	0.5	2.0
A9	1/2MS	0.5	3.0
A10	1/2MS	1.0	1.0
A11	1/2MS	1.0	2.0
A12	1/2MS	1.0	3.0

处理	基本培养基	NAA/(mg/L)	6-BA/(mg/L)
A1	MS	0.5	1.0
A2	MS	0.5	2.0
A3	MS	0.5	3.0
A4	MS	1.0	1.0
A5	MS	1.0	2.0
A6	MS	1.0	3.0
A7	1/2MS	0.5	1.0
A8	1/2MS	0.5	2.0
A9	1/2MS	0.5	3.0
A10	1/2MS	1.0	1.0
A11	1/2MS	1.0	2.0
A12	1/2MS	1.0	3.0

处理	基本培养基	NAA/(mg/L)	6-BA/(mg/L)
B1	MS	0.2	1.0
B2	MS	0.5	2.0
B3	MS	1.0	3.0
B4	1/2MS	0.2	1.0
B5	1/2MS	0.5	2.0
B6	1/2MS	1.0	3.0

处理	基本培养基	NAA/(mg/L)	6-BA/(mg/L)
B1	MS	0.2	1.0
B2	MS	0.5	2.0
B3	MS	1.0	3.0
B4	1/2MS	0.2	1.0
B5	1/2MS	0.5	2.0
B6	1/2MS	1.0	3.0

处理	基本培养基	NAA/(mg/L)	IBA/(mg/L)
C1	MS	0.5	0.5
C2	MS	0.5	1.0
C3	MS	0.5	1.5
C4	1/2MS	1.0	0.5
C5	1/2MS	1.0	1.0
C6	1/2MS	1.0	1.5