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中国农学通报 ›› 2024, Vol. 40 ›› Issue (24): 133-142.doi: 10.11924/j.issn.1000-6850.casb2023-0834

• 植物保护·农药 • 上一篇    下一篇

寡糖联合淡紫紫孢菌对化学杀线剂防治烤烟根结线虫病效果的影响

赵小明1(), 张雪慧2,3, 代快4(), 李江舟4, 曹海龙2, 徐爽2, 张翠萍4, 薛勐4, 崔永和4, 尹恒1()   

  1. 1 中国科学院大连化学物理研究所,辽宁大连 116023
    2 中科禾一(大连)农业科技有限公司,辽宁大连 116039
    3 沈阳农业大学植物保护学院,沈阳 110866
    4 云南省烟草公司玉溪市公司,云南玉溪 653100
  • 收稿日期:2023-12-08 修回日期:2024-04-27 出版日期:2024-08-20 发布日期:2024-08-20
  • 通讯作者:
    尹恒,男,1982年出生,湖北黄石人,研究员,博士,研究方向:糖生物学。通信地址:116023 辽宁省大连市沙河口区中山路457号,Tel:0411-84379061,E-mail:
    代快,女,1984年出生,湖南汉寿人,高级农艺师,博士,研究方向:烟草营养与病害防治。通信地址:653100 云南省玉溪市红塔区凤凰路102号,Tel:0877-2966641,E-mail:
  • 作者简介:

    赵小明,男,1963年出生,陕西杨陵人,正高级工程师,博士,研究方向:糖生物学。通信地址:116023 辽宁省大连市沙河口区中山路457号,Tel:0411-84379061,E-mail:

  • 基金资助:
    中国烟草总公司云南省烟草公司科技计划重点项目“连作土壤生物肥力和烟草抗病性提升关键技术研究应用”(2021530000241032); 陕西省重点研发计划重点产业创新链(群)项目“设施蔬菜安全优质生产生态调控技术集成及示范”(2020ZDLNY07-06)

Effect of Oligosaccharides Combined with Purpureocillium lilacinum on Efficacy of Chemical Nematicides on Flue-cured Tobacco Root-knot Nematode Disease

ZHAO Xiaoming1(), ZHANG Xuehui2,3, DAI Kuai4(), LI Jiangzhou4, CAO Hailong2, XU Shuang2, ZHANG Cuiping4, XUE Meng4, CUI Yonghe4, YIN Heng1()   

  1. 1 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023
    2 Zhongke Heyi (Dalian) Agricultural Technology Co., Ltd., Dalian, Liaoning 116039
    3 College of Plant Protection, Shenyang Agricultural University, Shenyang 110866
    4 Yunnan Tobacco Company Yuxi Branch, Yuxi, Yunnan 653100
  • Received:2023-12-08 Revised:2024-04-27 Published:2024-08-20 Online:2024-08-20

摘要:

为更好地控制烟草根结线虫的危害,降低化学杀线剂的用量,将寡糖免疫诱导剂与噻唑膦、阿维菌素及淡紫紫孢菌协同使用进行田间小区试验,研究其对烟草根结线虫的防治效果。结果表明,壳寡糖15 L/hm2、几丁寡糖15 L/hm2分别与噻唑膦22.5 kg/hm2协同使用,对烟草根结线虫的防治效果高于噻唑膦单独使用效果。相对防效由噻唑膦单独使用的45.20%分别提高到64.42%和65.38%。进一步试验发现,几丁寡糖1 kg/hm2、淡紫紫孢菌15 kg/hm2及噻唑膦16.875 kg/hm2(减少25%)协同使用,对烟草根结线虫病相对防效及对烟草生长总体效应优于与噻唑膦正常用量(22.5 kg/hm2)组和噻唑膦减少50%(11.25 kg/hm2)组合效果。寡糖诱导剂单独或与淡紫紫孢菌协同使用,显著提高了噻唑膦对烟草根结线虫病的防治效果,减少了农药使用量,为烟草根结线虫病害的防控提供了一种新的技术手段。

关键词: 烟草根结线虫病, 寡糖植物免疫诱导剂, 淡紫紫孢菌, 噻唑膦, 防治效果, 协同防治, 农药减量

Abstract:

In order to better control the harm of tobacco root-knot nematode and reduce the dosage of chemical nematocides, oligosaccharide immunoinducer was used together with fosthiazate, avermectin and Purpureocillium lilacinum in field plot experiment to study the control effect on tobacco root-knot nematode (RKN). The results in 2021 showed that the synergistic application of 15 L/hm2 chitosan oligosaccharides combined with 22.5 kg/hm2 fosthiazate, or 15 L/hm2 chitin oligosaccharides combined with 22.5 kg/hm2 fosthiazate had a significantly higher control effect to RKN than that of fosthiazate alone. The relative control effect of chitosan oligosaccharides combined with fosthiazate, chitin oligosaccharides combined with fosthiazate treatment was 64.42% and 65.38%, respectively, and that of fosthiazate treatment was 45.20%. These two treatments significantly promoted the growth of flue-cured tobacco. The results in 2022 showed that when 1 kg/hm2 chitin oligosaccharides, 15 kg/hm2 P. lilacinum and 16.875 kg/hm2 fosthiazate (25% reduction) were used together, the relative control effect to RKN and overall effect on tobacco growth was better than that of normal amount (22.5 kg/hm2) and 50% reduction of fosthiazate (11.25 kg/hm2). Therefore, the synergistic application of oligosaccharide with fosthiazate, and oligosaccharide with P. lilacinum and fosthiazate can improve the control effect of tobacco root-knot nematode disease and reduce the amount of chemical nematicides, and which can be used as one of the effective control technologies for plant nematode disease control.

Key words: tobacco root-knot nematodes disease, oligosaccharide plant immune inducer, Purpureocillium lilacinum, fosthiazate, control effect, integrated pest management, pesticide reduction