欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (11): 84-91.doi: 10.11924/j.issn.1000-6850.casb2025-0389

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

麻栗坡野生叠鞘石斛天然孕育小苗根际微生物多样性分析

彭翠仙1(), 王灿1, 杨玉玲1, 李桂琳2, 李玲1, 宋阳梅3, 赵大伟1, 陶永宏1()   

  1. 1 云南省文山州农业科学院， 云南文山 663000
    2 云南省德宏热带农业科学研究所， 云南瑞丽 678600
    3 云南省文山州三七和中医药产业发展中心， 云南文山 663000
  • 收稿日期:2025-05-09 修回日期:2026-04-17 出版日期:2026-06-12 发布日期:2026-06-12
  • 通讯作者:
    陶永宏，男，1982年出生，云南泸西人，研究员，博士，研究方向：中药材种质资源遗传育种研究。通信地址：663000 云南省文山壮族苗族自治州文山市泰康西路2号，Tel：0876-3037538，E-mail：。
  • 作者简介:

    彭翠仙，女，1988年出生，云南曲靖人，农艺师，硕士，研究方向：中药资源收集及开发利用研究。通信地址：663000 云南省文山壮族苗族自治州文山市泰康西路2号，Tel：0876-3037538，E-mail：。

  • 基金资助:
    云南省科技特派队“麻栗坡县下金厂乡铁厂乡科技特派队”(WS031020)

Analysis of Microbial Diversity in Rhizosphere Soil of Naturally Nurturing Seedlings of Dendrobium denneanum in Malipo

PENG Cuixian1(), WANG Can1, YANG Yuling1, LI Guilin2, LI Ling1, SONG Yangmei3, ZHAO Dawei1, TAO Yonghong1()   

  1. 1 Wenshan Academy of Agricultural Sciences, Wenshan, Yunnan 663000
    2 Yunnan Dehong Institute of Tropical Agriculture, Ruili, Yunnan 678600
    3 Panax notoginseng and Traditional Chinese Medicine Industry Development Center, Wenshan, Yunnan 663000
  • Received:2025-05-09 Revised:2026-04-17 Published:2026-06-12 Online:2026-06-12

RichHTML

1

PDF (PC)

2

摘要：

本研究旨在探究麻栗坡叠鞘石斛小苗天然孕育环境及根际微生物状况，为揭示其自然孕育机制提供理论基础。通过高通量测序技术，分析了一年生小苗根际土壤与附近未孕育小苗土壤的微生物多样性、组成结构差异。结果表明：与未孕育石斛小苗的土壤相比较，一年生石斛小苗根际土壤中细菌和真菌群落多样性显著降低。整体上看细菌门水平下丰度第1的是变形菌门Proteobacteria (SCK：29.48%，A1：40.23%)，第2是拟杆菌门Bacteroidota (SCK：33.73%，A1：19.12%)、排名第3和第4的分别是酸杆菌门Acidobacteriota和浮霉菌门Planctomycetota；真菌中丰度第1的是子囊菌门Ascomycota (SCK：45.23%，A1：37.43%)，第2的是未分类被孢霉门Mortierellomycota (SCK：31.89%，A1：48.35%)，第3和第4的分别是原生动物纤毛虫门Ciliophora和担子菌门Basidiomycota。差异分析发现2个样本土壤微生物群落结构组成存在显著差异（细菌：R2=0.900、P=0.001；真菌：R2=0.963、P=0.001）。土壤环境因子如有机质、总氮、Ca等与有益菌如被孢霉属Mortierella、鞘氨醇单胞菌属Sphingomonas等有益菌存在显著关联。研究结果可为叠鞘石斛种子萌发相关微生物资源开发，以及兰科植物与微生物之间相互作用分析提供科学依据。

关键词: 叠鞘石斛, 天然小苗, 孕育环境, 根际微生物, 多样性

Abstract:

To investigate the natural germination environment and rhizosphere microbial status of Dendrobium denneanum seedlings in Malipo, high-throughput sequencing was employed to analyze the microbial diversity and community composition differences between the rhizosphere soil of one-year-old seedlings and nearby non-germinated seedling soil. The results indicated that compared to non-germinated Dendrobium denneanum seedling soil, the diversity of bacterial and fungal communities in one-year-old Dendrobium denneanum seedling rhizosphere soil significantly decreased. At the phylum level, Proteobacteria ranked the first in bacterial abundance (SCK: 29.48%, A1: 40.23%), followed by Bacteroidota (SCK: 33.73%, A1: 19.12%), with Acidobacteriota and Planctomycetota occupying the third and fourth positions, respectively. Among fungi, Ascomycota showed the highest abundance (SCK: 45.23%, A1: 37.43%), followed by unclassified Mortierellomycota (SCK: 31.89%, A1: 48.35%), while Ciliophora and Basidiomycota ranked third and fourth. Variation analysis revealed that significant differences were found in microbial community composition between the two soil samples (bacteria: R2=0.900, P=0.001; fungi: R2=0.963, P=0.001). Soil environmental factors such as organic matter, total nitrogen, and Ca exhibited significant correlations with beneficial microbes like Mortierella and Sphingomonas. These findings provide scientific insights for developing microbial resources related to Dendrobium denneanum seed germination and analyzing interactions between orchid plants and microorganisms.

Key words: Dendrobium aurantiacum, natural seedlings, nurturing environment, rhizosphere microorganisms, diversity

中图分类号: