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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (4): 85-88.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

正交设计在刺五加组织培养中的应用

孙周平1,褚丽敏2,洪仁伟3   

  • 收稿日期:2009-09-14 修回日期:2009-10-17 出版日期:2010-02-20 发布日期:2010-02-20
  • 基金资助:

    辽宁省“十一五”重大项目资助

Application of Orthogonal Design in Experiments on Tissue Culture of Acanthopanax Senticosus

  • Received:2009-09-14 Revised:2009-10-17 Online:2010-02-20 Published:2010-02-20

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摘要：

以刺五加不定芽为外植体，采用二次回归正交旋转设计方法，对刺五加初代培养中6-BA和NAA的浓度配比进行筛选。通过对所建模型的分析得出：6-BA 的最优浓度为0.72-1.28 mg?L-1，NAA的最优浓度为0.05-0.15 mg?L-1，其可信度达95％。

关键词: 左旋咪唑, 左旋咪唑, 抗体, 新城疫疫苗

Abstract:

Quadratic regressive orthogonal revolving design experiment was used to select optimum concentration of 6-BA and NAA in WPM medium for shoot propagation of Acanthopanax senticosus．The result showed that the most of shoots achieved by the 6-BA and NAA concentrations were in a rang of 0.72-1.28 mg?L-1 and 0.05-0.15 mg?L-1 respectively．The probability of the event was 95％．