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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (15): 297-300.

• 植物保护 农药 • 上一篇    

瑞香狼毒超临界CO2萃取物的杀螨毒力及机理初探

梁为 马兰青 卜春亚 成军 靳永胜 王有年 师光禄   

  • 收稿日期:2011-01-10 修回日期:2011-02-10 出版日期:2011-06-25 发布日期:2011-06-25
  • 基金资助:

    核桃青皮杀螨活性成分及作用机理研究;有机观光果园间作对有害生物及生态环境影响的研究;北京山区果品优质生态安全关键技术研究与示范;核桃青皮杀螨活性物质及作用毒理学

Preliminary Study on Toxicity of Supercritical Extract of Stellera chamaejasme L. to Tetranychus cinnabarinus and its Acaricidal Mechanism

  • Received:2011-01-10 Revised:2011-02-10 Online:2011-06-25 Published:2011-06-25

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摘要：

为了测定瑞香狼毒超临界CO2萃取物对朱砂叶螨的触杀毒力，并了解其作用机理，笔者采用玻片浸渍法测定瑞香狼毒超临界CO2萃取物（SCE）对朱砂叶螨的触杀毒力，观察其对朱砂叶螨的致毒症状；结果表明：SCE对朱砂叶螨24 h的触杀LC50值为2.411 mg/mL；中毒试螨表现出类似神经毒剂的致毒症状，如兴奋、痉挛等。随后测定了SCE对朱砂叶螨神经系统靶标酶（乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶）活性的影响。结果表明，SCE能够显著抑制试螨乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性；据此推测，SCE对朱砂叶螨可能具有神经毒性。

关键词: 人工湿地, 人工湿地, 农业径流, 处理

Abstract:

In order to study the toxicity and possible lethal mechanism of supercritical extract of Stellera chamaejasme L., the experiment was conducted. In this study, the contact toxicity of supercritical extract of Stellera chamaejasme L. (SCE) to Tetranychus cinnabarinus was tested using slide dip method, and dynamic toxicosis symptoms of SCE-exposed mites were also detailedly observed. The results showed that the contact toxicity LC50 value of SCE to T. cinnabarinus was 2.411 mg/mL at 24th h post-treatment. Some typical neurotoxic symptoms such as excitement and convulsions were observed in SCE-exposed mites. Subsequently, the changes of activities of AChE, MAO, Na+-K+-ATPase and Ca2+-Mg2+-ATPase after exposed to SCE were assayed. The experiment showed that SCE could significantly inhibit the activities of AChE, MAO, Na+-K+-ATPase and Ca2+-Mg2+-ATPase. The results led us to believe that SCE may be a neurotoxin.

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