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中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 200-204.

• 林学 园艺 园林 • 上一篇    下一篇

白芦笋小孢子发育时期与花器形态相关性研究

林宗铿 张天翔 杨俊杰 蔡坤秀 陈振东 林义章   

  • 收稿日期:2011-06-09 修回日期:2011-07-26 出版日期:2011-11-05 发布日期:2011-11-05
  • 基金资助:

    福建省自然科学基金项目“利用游离小孢子培育芦笋超雄株技术研究”

Correlation of Microspore Development Period and the Flower Organ Morphology of White Asparagus

  • Received:2011-06-09 Revised:2011-07-26 Online:2011-11-05 Published:2011-11-05

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摘要：

为了寻找白芦笋小孢子发育时期的鉴定方法以及探讨小孢子发育时期与花器形态的相关性，采用苯酚品红、醋酸洋红和醋酸铁矾水合三氯乙醛苏木精染色液进行染色观测，并对白芦笋小孢子发育时期的细胞学以及小孢子不同发育时期与花蕾大小的关系进行了研究。结果表明，醋酸铁矾水合三氯乙醛苏木精染色效果最好；白芦笋小孢子发育经四分体时期、单核早中期、单核靠边期和双核期，各时期的特征明显；白芦笋小孢子发育时期与花蕾的外部形态特征密切相关，不同发育时期的花蕾其纵径、横径间差异显著，白芦笋品种的小孢子处于单核靠边期时花蕾纵径为2.30 mm，花蕾横径为1.76 mm；依据白芦笋花蕾的纵径和横径可判断小孢子的发育时期，从而确定小孢子培养最佳时期所对应的选蕾标准。

关键词: 可乐猪, 可乐猪, 贵州白香猪, apoA5基因, 比较分析

Abstract:

The purpose of this paper is to find a identification method of the developmental asparagus microspore and discuss the relevance between the developmental period of asparagus microspore and the morphology of flower organ. So the phenol magenta, carmine acetate and heidenhain iron hematoxylin solutions were selected for use in staining observation to asparagus microspore, then the developmental cytology, the relevance between the microspore development and the bud’s size have been studied. The results showed that the effect of heidenhain iron hematoxylin was the best in the three solutions. The development of asparagus microspore was divided into tetrad, early- or mid-uninucleate, late-uninucleate and binucleate stages, each stage demonstrated distinctive characters. The developmental periods of asparagus microspore were closely related to the morphological characters of buds. The longitudinal diameter and transverse diameter of buds had significant differences in different developmental periods. When the asparagus microspores were at the late-uninucleate, the buds showed 2.30 mm in longitudinal diameter and 1.76 mm in transverse diameter. The developmental period of asparagus microspore could be judged by the longitudinal diameter and transverse diameter of buds, so as to define the standard of bud selection corresponding with the optimal stage of microspore culture.