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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 205-210.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

60Co-γ射线对芹菜种子的辐射效应

阎君 于力 褚云霞 许爽 朱为民   

  • 收稿日期:2011-06-16 修回日期:2011-08-25 出版日期:2011-11-05 发布日期:2011-11-05

Effect of 60Co-γ Ray Irradiation on Celery Seed

  • Received:2011-06-16 Revised:2011-08-25 Online:2011-11-05 Published:2011-11-05

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摘要：

以3个芹菜品种的干种子为材料，用不同剂量的60Co-γ射线进行辐照处理，研究辐照对种子活力和M1代苗期生长及生理指标的影响。结果表明，辐射对3个品种的效应一致。当辐照剂量达到或高于200 Gy时，芹菜种子的萌发能力和种子活力显著下降，当辐照剂量为300 Gy时，种子没有发芽能力。60Co-γ射线辐照影响了芹菜M1代幼苗的生长和生理代谢，当辐照剂量达到或大于200 Gy时，幼苗的株高下降了50%~60%；50、100、150 Gy的辐照剂量刺激了幼苗的超氧化物歧化酶（SOD）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）和谷光甘肽还原酶（GR）活性的增加，50、100、150 Gy的辐照剂量下超氧阴离子（O2ˉ?）产生速率和丙二醛（MDA）的含量和对照差异不显著，当辐照剂量达到或超过200 Gy时，幼苗SOD、POD和APX活性显著下降，且O2ˉ?产生速率和MDA含量显著上升，说明低于或等于150 Gy的辐照不会对芹菜幼苗的生长造成影响。综合分析所测定的指标，芹菜干种子的适宜诱变剂量为100~150 Gy。

关键词: 土地适宜性, 土地适宜性, 机械化, 传统耕作, 尺度推绎, 土地单元

Abstract:

The dry seeds of celery were irradiated by different doses of 60Co-γ ray, the effects of radiation on seed vigor and M1 seedlings physiological characteristics were investigated. The results showed the effect of 60Co-γ ray irradiation on three cultivars seeds were similar. When the doses of 60Co-γ ≥200 Gy, the germination and vigor of seed decreased remarkably. And there was no germination after irradiated by 300 Gy 60Co-γ. There was a negative effect of radiation on M1 seedlings. The M1 seedlings height declined with 50%-60% when the doses of 60Co-γ ≥200 Gy. When the doses were 50, 100, 150 Gy, the SOD, POD, APX, GR activity increased compare with 0 Gy , and there was not remarkable difference on O2ˉ? production rate and MDA content between 50, 100, 150 Gy 60Co-γ and 0 Gy 60Co-γ. However, when the doses of 60Co-γ ≥200 Gy, the SOD, POD, APX activity decreased remarkably and O2ˉ? production rate, MDA content increased remarkably. The 60Co-γ ≤150 Gy did not inhibit the growth of celery seedlings. Therefore, we concluded that the optimum doses of celery dry seeds was 100-150 Gy.