欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 117-123.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

微卫星荧光标记基因分型实验误差控制

徐 绯 袁俊霞 赵 致 潘 锦   

  • 收稿日期:2010-11-02 修回日期:2010-12-28 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    科技部国家重点基础研究发展计划973;国家自然科学基金青年基金项目

Limitation to Genotyping Errors of Fluorescent Microsatellite

  • Received:2010-11-02 Revised:2010-12-28 Online:2011-03-20 Published:2011-03-20

PDF (PC)

1372

摘要：

分子标记基因分型误差对研究结果的影响越来越为研究者们所关注。本研究以茄子（Solanum melongena L.）野生和栽培类群为研究材料，从微卫星荧光基因分型中PCR扩增实验体系和荧光标记判读两个环节控制实验的误差率，总结出一套简便、快速的微卫星分子标记PCR扩增实验优化流程；并且，针对微卫星荧光标记基因分型中存在的各种误差，提出相应的分析方法和检测方案，为利用微卫星分子标记技术的研究提供实验操作依据和数据处理参考。

关键词: 双向电泳, 双向电泳

Abstract:

More and more researchers focus on genotyping errors because of their effect on the biological conclusions of a study. Using the fluorescent microsatellite genotyping of wild and cultivated eggplants (Solanum melongena L.) as example, error rates had been limited from these two processes that are PCR (polymerase chain reaction) amplification and identification of the fluorescence peak in this study. The rapid and simple protocol was created for optimizing PCR amplification conditions. And some strategies were proposed to discern and assess errors from the fluorescence data. Our study provides some reference for experiment operation and data handling of microsatellite markers.