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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (1): 176-180.doi: 10.11924/j.issn.1000-6850.2011-2258

所属专题： 油料作物； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

甘蓝型油菜高油酸材料的遗传分析

费维新 吴新杰 李强生 陈凤祥 侯树敏 江莹芬 荣松柏 胡宝成   

  • 收稿日期:2011-08-03 修回日期:2011-10-24 出版日期:2012-01-05 发布日期:2012-01-05
  • 基金资助:

    油菜高油酸性状的遗传与分子标记研究

Gentetic Analysis of High Oleic Acid Mutation materials in Brassica napus

  • Received:2011-08-03 Revised:2011-10-24 Online:2012-01-05 Published:2012-01-05

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摘要：

为探索甘蓝型油菜突变自交系高油酸性状的遗传模式，以应用于油菜高油酸育种工作。本研究以低油酸亲本‘扬6026’（P1）和甘蓝型油菜高油酸突变自交系‘HO05’（P2）为材料，通过气相色谱单粒法分析对该组合的杂交后代F1、RF1、B1、RB1、B2、RB2、F2和RF2的高油酸性状的进行数量性状遗传分析。试验分析结果表明：亲本的油酸含量比较稳定，为加性-显性遗传模式，并且加性主效基因作用明显，是由于基因遗传差异作用的，高油酸性状由2对主效基因控制高油酸性状，狭义遗传力hN2为63.16%，另外的36.84%是由环境的变化引起的；低油酸亲本（P1）和高油酸亲本（P2）正反交试验表明，高油酸的遗传没有细胞质遗传效应。

关键词: 美洲黑杨, 美洲黑杨, ISSR-PCR, 多态位点, 遗传距离

Abstract:

To search for the genetic model of high oleic acid mutations in Brassica napus for high oleic acid-breeding, this study used a low oleic acid content line ‘Yang6026’ (P1) and a high oleic acid content mutation line ‘HO05’ (P2) and their crossing Progenies (F1, RF1, B1, RB1, B2, RB2, F2 and RF2) to analyze the quantitative genetic patterns of high oleic acid. The content of oleic acid was measured by gas chromatograph using single seed method. The results show that, the oleic acid content of parents were stable; Inheritance of high oleic acid was controlled by 2 major genes and present additive-dominance genetic model, and additive-major gene effect was distinct. Two reciprocal cross (F1, RF1) of the two parents (P1, P2) revealed that high oleic acid was non-cytoplasmic . Inheritance Heritability in narrow sense (hN2)of high oleic acid was 63.16% which caused by genotype , and 36.84% was caused by environment.