家蚕核型多角体病毒对家蚕酚氧化酶活性及其基因表达的影响

doi:10.11924/j.issn.1000-6850.casb16050089

中国农学通报 ›› 2016, Vol. 32 ›› Issue (32): 25-28.doi: 10.11924/j.issn.1000-6850.casb16050089

所属专题：生物技术

家蚕核型多角体病毒对家蚕酚氧化酶活性及其基因表达的影响

唐芬芬^1,2,杨伟克¹,朱峰¹,邵榆岚¹,张永红¹,白兴荣¹

(¹云南省农业科学院蚕桑蜜蜂研究所,云南蒙自 661101；²云南农业大学植物保护学院,昆明 650201）

收稿日期:2016-05-16 修回日期:2016-11-02 接受日期:2016-06-20 出版日期:2016-11-15 发布日期:2016-11-15
通讯作者: 唐芬芬
基金资助:
云南省现代农业蚕桑产业技术体系建设项目“蚕桑遗传育种研究室经费”(2013KJ-TX006))；国家自然科学基金项目“云南蚕区BmNPV流行株系鉴定及交互保护”(31260583)。

BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori

Tang Fenfen^1,2, Yang Weike¹, Zhu Feng¹, Shao Yulan¹, Zhang Yonghong¹, Bai Xingrong¹

(¹Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101;²College of Plant Protection, Yunnan Agricultural University, Kunming 650201)

Received:2016-05-16 Revised:2016-11-02 Accepted:2016-06-20 Online:2016-11-15 Published:2016-11-15

摘要/Abstract

摘要： 为明确家蚕核型多角体病毒(BmNPV)感染对家蚕血淋巴酚氧化酶活性及其基因表达的影响，以5龄起蚕为实验材料，经口添食BmNPV，采用实时荧光定量PCR的方法检测了血淋巴酚氧化酶原基因PPO1和PPO2的表达情况，同时测定了酚氧化酶的活性。结果表明，PPO1和PPO2基因均是在添食BmNPV后6 h和9 h出现上调表达，但相对表达量有一定差异；另外，酚氧化酶活性在添食BmNPV后 6 h和9 h显著高于对照组，而在24 h急剧下降，显著低于对照组。酚氧化酶作为一类体液免疫因子，很可能参与了家蚕对抗BmNPV侵染的免疫防御反应。

关键词: 小波分析, 小波分析, 空气污染指数, 时间尺度, 成都市

Abstract: In order to clarify the effect of BmNPV infection on the activity and gene expression of phenoloxidase in hemolymph of Bombyx mori, the real-time fluorescent quantitative PCR was applied to analyze the expression of prophenoloxidase gene PPO1 and PPO2 in hemolymph of the 5^th instar larvae of silkworm, meanwhile the activity of phenoloxidase was determined after feeding Bombyx mori nucleopolyhedrovirus. The results revealed that the expression of PPO1 and PPO2 presented an increasing trend after oral infected by BmNPV after 6 h and 9 h, but the relative expression quantity of PPO1 had significant difference with that of PPO2 at 6 h and 9 h. In addition, the phenoloxidase activities of the treatment group were significantly higher than that of the control group at 6 h and 9 h, while phenoloxidase activity of the treatment group was significantly lower at 24 h. As a kind of humoral immunity factor, phenoloxidase may be involved in the immune defense response of silkworm by BmNPV infection.

中图分类号:

S884.5
Q789

唐芬芬,杨伟克,朱峰,邵榆岚,张永红,白兴荣. 家蚕核型多角体病毒对家蚕酚氧化酶活性及其基因表达的影响[J]. 中国农学通报, 2016, 32(32): 25-28.

Tang Fenfen,Yang Weike,Zhu Feng,Shao Yulan,Zhang Yonghong and Bai Xingrong. BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori[J]. Chinese Agricultural Science Bulletin, 2016, 32(32): 25-28.

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家蚕核型多角体病毒对家蚕酚氧化酶活性及其基因表达的影响

BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori

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