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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (7): 55-60.doi: 10.11924/j.issn.1000-6850.casb2024-0539

• 林学·园艺·园林 • 上一篇    下一篇

瓶内接种AM真菌对牡丹组培苗生长发育的影响

曾端香1(), 于宁1(), 杜娟1, 曾瑶1, 王莲英2   

  1. 1 国家林业和草原局管理干部学院，北京 102600
    2 北京林业大学园林学院，北京 100083
  • 收稿日期:2024-08-27 修回日期:2025-01-23 出版日期:2025-03-05 发布日期:2025-03-03
  • 通讯作者:
    于宁，男，1981年出生，山东寿光人，助理研究员，硕士，研究方向：农业推广、职业教育。通信地址：102600 北京市大兴区林校北路8号 国家林业和草原局管理干部学院，E-mail：。
    王莲英，女，1936年出生，河南开封人，教授，研究方向：园林植物栽培与应用。Tel：010-62337525。
  • 作者简介:

    曾端香，女，1972年出生，湖南邵东人，教授，博士，研究方向：园林植物栽培繁育与应用、园林规划设计、菌根生态研究。通信地址：102600 北京市大兴区林校北路8号 国家林业和草原局管理干部学院，E-mail：。

  • 基金资助:
    国家林业和草原局“百千万人才工程”省部级人选(2020); 国家林业和草原局管理干部学院重点课题“全国林草职业院校科技成果转化路径研究”(202315)

Effects of Arbuscular Mycorrhiza Fungi Inoculated in Vitro on Growth and Development of Tree Peony Vitroplantlets

ZENG Duanxiang1(), YU Ning1(), DU Juan1, ZENG Yao1, WANG Lianying2   

  1. 1 National Academy of Forestry and Grassland Administration, Beijing 102600
    2 College of Landscape Architecture, Beijing Forestry University, Beijing 100083
  • Received:2024-08-27 Revised:2025-01-23 Published:2025-03-05 Online:2025-03-03

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摘要：

为探索丛枝菌根真菌（AM真菌）对牡丹组培苗菌根化过程和生长发育的影响，以‘乌龙捧盛’牡丹的休眠芽组培的根诱导苗和生根苗以及杂交系‘Z1-WM’和‘Z1-10-3’的胚培养而得的胚根苗为实验材料，在组织培养试管瓶内接种不同种类的AM真菌孢子。结果表明，AM真菌能显著促进牡丹菌根化组培苗的生长发育，瓶内接种AM真菌比对照（未接种）能更有效地促进牡丹上胚轴萌发率，最高达99.5%，且比对照提前30 d以上打破上胚轴休眠，极显著高于GA3、2~4℃低温和对照处理。接种Gg+Gm对‘Z1-10-3’和‘Z1-WM’组培苗的生长增效最显著；接种Gg对‘乌龙捧盛’组培苗的增效作用最大，移栽6个月时的株高、根长、地上部干重、地下部干重、叶面积增长率分别为64.93%、70.90%、35.00%、54.55%、41.15%。因此，AM真菌对牡丹菌根化组培苗生长发育有极显著的增效作用，且缩短了育种进程。

关键词: 牡丹, 瓶内接种, AM真菌, 丛枝菌根, 菌根化组培苗, 打破上胚轴休眠, 生长发育

Abstract:

To explore arbuscular mycorrhiza (AM) fungi of mycorrhization process and the impact on its growth and development, with tree peony cultivar ‘Wulong Pengsheng’ for bud-micropropagated vitroplantlets of rooting seedlings and hybrid system ‘Z1-WM’ and ‘Z1-10-3’ embryo culture rooting plantlets as the research objects, different kinds of AMF spores in vitro were inoculated. The results showed that active effects of AMF on the growth of mycorrhizal vitroplantlet were investigated. Inoculation of AMF in vitro could increase the transplanting survival ratio of tree peony effectively. The treatments of inoculation of AMF in vitro heightened sprouting rate epicotyl dormancy of plantlets more than CK, and the highest was 99.50%. The time of breaking epicotyl dormancy was 30 d ahead of inoculation of AMF in vitro than that of the control. There was significant difference among inoculation of AMF in vitro and GA3 treatment, 2-4℃ low temperature and the control. The most significant difference was in ‘Z1-WM’ and ‘Z1-10-3’ inoculated with Gg+Gm. As for ‘Wulong Pengsheng’, after transplantation of 6 months inoculated with Gg, the index was 64.93%, 70.90%, 35.00%, 54.55%, 41.15% respectively. Therefore, AM fungi had a significant effect on the growth and development of mycorrhized vitroplantlets of tree peony and shorten the breeding time.

Key words: tree peony, inoculated in vitro, AM fungi, arbuscular mycorrhiza, mycorrhized vitroplantlets, breaking epicotyl dormancy, growth and development