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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (15): 102-105.

所属专题： 生物技术； 马铃薯

• 农学 农业基础科学 • 上一篇    下一篇

甘薯叶片和叶柄组织诱导培养及植株再生研究

徐茜 乐正碧 徐燕 文明玲 王良平 余鸿燕 黎华 张菡   

  • 收稿日期:2010-11-08 修回日期:2010-12-14 出版日期:2011-06-25 发布日期:2011-06-25

Study of Leaf and Petiole Tissue Culture Induction and Plant Regeneration on Sweet Potato

  • Received:2010-11-08 Revised:2010-12-14 Online:2011-06-25 Published:2011-06-25

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摘要：

为探索甘薯叶片和叶柄组织诱导和植株再生技术，应用甘薯优良品种‘万薯7号’叶片、叶柄在7个诱导培养基中的离体培养。试验表明，‘万薯7号’叶片和叶柄在本试验的7个诱导培养基中，极易诱导产生愈伤组织，诱导率达100%。叶片和叶柄在经培养基MS+2.0 mg/L KT+0.5 mg/L IAA诱导出绿色愈伤组织后，在继代培养基MS+4.0 mg/L 6-BA+0.01 mg/L NAA培养15天后，均诱导成苗。

关键词: 添加剂, 添加剂

Abstract:

It was studied the leaf and petiole tissue culture induction and plant regeneration with the sweet potato variety ‘Wanshu No. 7’. The result indicated the sweet potato variety of leave and petiole could be easy to induce callus in 7 induction mediums, the induction rate of 100%. The leaf and petiole induced green callus in the medium of MS +2.0 mg/L KT +0.5 mg/L IAA, and then subculture 15 days in the medium of MS +4.0 mg/L 6-BA + 0.01 mg/L NAA , they were induced emergence seeding.