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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (20): 76-78.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸡源性大肠杆菌产超广谱β-内酰胺酶基因型分布

张利勃 刘延庆 高慎阳   

  • 收稿日期:2011-01-20 修回日期:2011-05-05 出版日期:2011-08-15 发布日期:2011-08-15

Genotype Distribution of Extended-Spectrum β-Lactamases Produced by Escherichia coli Isolated from the Fowl in Dalian Area of Liaoning

  • Received:2011-01-20 Revised:2011-05-05 Online:2011-08-15 Published:2011-08-15

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摘要：

摘要: 检测鸡大肠杆菌超广谱β-内酰胺酶（ESBLs）的基因型并了解辽宁省鸡源性大肠杆菌产ESBLs基因型分布。对大连4个地区不同鸡场临床分离32株产ESBLs鸡大肠杆菌分别用TEM、OXA、CTX-M和SHV等4种通用引物进行PCR扩增确定其基因型。结果显示32株鸡大肠杆菌均检出TEM型基因、12株检出CTX-M型基因、2株检出OXA型、未检出SHV型；TEM+CTX-M双阳性检出11株、TEM+OXA双阳性检出2株。TEM基因亚型占检出比例最高（100％）。结论：目前辽宁省产ESBLs鸡大肠杆菌基因亚型以TEM为主，其次为CTX-M型，未发现SHV型；以TEM+CTX-M双阳性检出率最高（34.4％）。

关键词: 麦田, 麦田

Abstract:

Abstract: Objective：to elucidate the genotype distributions of ESBLs produced by E.coli isolated from the fowl in Liaoning Province. Method: thirty-two ESBL-producing Escherichia coli were collected from four different chicken farms in Dalian, and were analyzed by PCR amplifying using the primers of TEM, OXA, SHV and CTX-M, respectively. Result: thirty-two strains with TEM-type, twelve CTX-M-types and two OXA-types were detected except SHV-type was not found; moreover, eleven strains with multiple TEM+CTX-M and two strains with TEM+OXA genotyes were detected. Conclusion: the predominant genotype of ESBLs is TEM (100% detection rate) in chicken farms of Liaoning Province, and then are CTX-M, SHV was not found; the multiple TEM+CTX-M positive rate reached up to 34.4％.

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