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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (19): 100-104.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

茶树一个冷诱导基因的克隆及其表达分析

李先文 余海波 孟琼 李峥峥 张新玉   

  • 收稿日期:2011-01-20 修回日期:2011-03-07 出版日期:2011-08-05 发布日期:2011-08-05
  • 基金资助:

    转热稳定蛋白酶基因茶树的创制

Clone and Expression Profile of A Cold-inducible Gene of Camellia sinensis

  • Received:2011-01-20 Revised:2011-03-07 Online:2011-08-05 Published:2011-08-05

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摘要：

研究茶树低温驯化的分子机制，不仅对阐明多年生植物抗冻性有重要的理论意义，也有助于茶树抗冻品种的选育。本研究运用RACE技术从茶叶中克隆出一个冷诱导基因CsCOR1的全长cDNA序列，并通过Real-time RT PCR方法分析了该基因在低温、高渗和外源ABA处理时的表达状况。结果显示，CsCOR1 cDNA包含一个推定的ORF，编码一86个氨基酸的多肽。该多肽由N-端的信号肽和富含甘氨酸、精氨酸和脯氨酸的C-端两部分组成，其成熟蛋白中有两个重复的13肽段。CsCOR1基因的表达可被低温和脱水胁迫剧烈上调，可被外源ABA中度上调。

关键词: 绵羊, 绵羊

Abstract:

Study about the mechanism of cold acclimation of tea plants is of importance for elucidating the frost resistance of perennial plants and breeding cold-resistance variety of tea plants. In present paper, CsCOR1 gene was cloned from tea leaves by RACE approach, and the expression profile of CsCOR1 gene were analyzed by Real-time RT PCR strategy under cold, hyperosmosis and ABA-treatment. The results show that CsCOR1 gene encodes a 86 amino-acid peptide, which consists of a hydrophobic N-terminal region for a signal peptide and a hydrophilic C-terminal domain that is rich in glycine，arginine and proline. There are two internal repeat sequences in mature CsCOR1. CsCOR1 expression was induced dramatically by cold/hyperosmosis and moderately by ABA in tea leaves via real-time RT PCR analysis.

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