TGF-β RⅠ及Smad2表达情况对绵羊腔前卵泡发育的影响

doi:10.11924/j.issn.1000-6850.casb16040076

中国农学通报 ›› 2016, Vol. 32 ›› Issue (32): 7-14.doi: 10.11924/j.issn.1000-6850.casb16040076

所属专题：畜牧兽医

TGF-β RⅠ及Smad2表达情况对绵羊腔前卵泡发育的影响

常迪,董晓晨,郭勇,刘云海,齐晓龙,盛熙晖,倪和民

(北京农学院动物科技学院,北京 102206）

收稿日期:2016-04-12 修回日期:2016-05-24 接受日期:2016-05-25 出版日期:2016-11-15 发布日期:2016-11-15
通讯作者: 倪和民
基金资助:
2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(PXM2013_014207_000067)。

Expression of TGF-β RⅠ and Smad2 Affecting the Development of Sheep Preantral Follicles

Chang Di, Dong Xiaochen, Guo Yong, Liu Yunhai, Qi Xiaolong, Sheng Xihui, Ni Hemin

(College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206)

Received:2016-04-12 Revised:2016-05-24 Accepted:2016-05-25 Online:2016-11-15 Published:2016-11-15

摘要/Abstract

摘要： 为探索适于哺乳动物腔前卵泡的培养方式以及观察颗粒细胞与卵母细胞的信号表达，为阐明早期卵泡生长的复杂机理和揭示卵泡发生发育的内在规律提供参考，通过使用免疫组织化学染色、荧光免疫组织化学染色以及RT-PCR的方法，观察TGF-β RⅠ与Smad2的蛋白和mRNA在绵羊卵巢卵泡的表达情况。结果表明：TGF-β RⅠ在绵羊腔前卵泡及小腔卵泡的颗粒细胞及卵母细胞有表达，随着卵泡的生长发育，到成熟卵泡时期时，信号消失；Smad2蛋白在绵羊卵泡各个发育时期的颗粒细胞中均有表达，在绵羊腔前卵泡的卵母细胞中无表达，随着卵泡的生长发育，信号逐渐出现，到成熟卵泡时期时，Smad2蛋白在卵母细胞表达较强；TGF-β RⅠ mRNA只在成熟卵泡和培养前腔前卵泡颗粒细胞中检测到表达，在成熟卵泡和培养前腔前卵泡颗粒细胞中均有Smad2 mRNA表达，但在培养后腔前卵泡颗粒细胞中Smad2 mRNA表达量较低。TGF-β RⅠ与Smad2的蛋白和mRNA表达与绵羊卵泡发育情况密切相关。

关键词: 区域生态安全格局, 区域生态安全格局, 肃州区, 景观格局, 景观安全格局, 系统耦合

Abstract: The aims of this study are to investigate the optimal way for cultivation of preantral follicles and observe the signal expression of granular cells and oocytes so as to explore the complex mechanism and inner law of folliculogenesis. The expressions of protein and mRNA of TGF-β RⅠ and Smad2 in sheep follicles were observed by using immunohistochemical staining, fluorescence immunohistochemical staining and RT-PCR. The results showed that TGF-β RⅠ signal expressed in granular cells and oocytes of sheep preantral follicles and small antral follicles but disappeared in mature follicles. Smad2 protein expressed in granular cells during all the development periods of sheep follicle, but did not express in oocytes of preantral follicles; with the development of follicle, the signal appeared and had a strong expression in oocytes of mature follicles. mRNA of TGF-β RⅠ only expressed in mature follicles and preantral follicles without culturing. mRNA of Smad2 expressed in all the follicles, but had a lower expression level in preantral follicles that were cultured for 6 days. The expression of protein and mRNA of TGF-β RⅠ and Smad2 have a close relation with the development of sheep follicles.

常迪,董晓晨,郭勇,刘云海,齐晓龙,盛熙晖,盛熙辉,倪和民. TGF-β RⅠ及Smad2表达情况对绵羊腔前卵泡发育的影响[J]. 中国农学通报, 2016, 32(32): 7-14.

Chang Di,Dong Xiaochen,Guo Yong,Liu Yunhai,Qi Xiaolong,Sheng Xihui, and Ni Hemin. Expression of TGF-β RⅠ and Smad2 Affecting the Development of Sheep Preantral Follicles[J]. Chinese Agricultural Science Bulletin, 2016, 32(32): 7-14.

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TGF-β RⅠ及Smad2表达情况对绵羊腔前卵泡发育的影响

Expression of TGF-β RⅠ and Smad2 Affecting the Development of Sheep Preantral Follicles

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