欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 149-152.

所属专题： 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

菊花白锈病抗性标准及人工离体接种鉴定方法的优化

祝朋芳 赵妮拉 齐丹 刘娜 段玉玺   

  • 收稿日期:2011-02-16 修回日期:2011-03-21 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    主要商品花卉优质高产新品种选育

Optimization of Identification Standards and Artificial Inoculation Methods in vitro on Resistance to Chrysanthemum White Rust

  • Received:2011-02-16 Revised:2011-03-21 Online:2011-06-05 Published:2011-06-05

PDF (PC)

951

摘要：

为使菊花白锈病的抗性鉴定在可控条件下进行，本文将目测观察与显微镜检方法相结合，制定了菊花白锈病6级抗性鉴定标准，并优化了人工离体接种方法。结果表明，培养瓶内茎段扦插法、培养皿内平铺叶片法均可用于菊花白锈病的离体鉴定；培养瓶内茎段扦插法优于培养皿内平铺叶片法，是最佳的人工离体接种鉴定方法。该优化方法是评价菊花白锈病抗性的既安全又简单的接种方法。

关键词: TMGMV, TMGMV, 三碱基组, 概率, K-M聚类

Abstract:

In order to identify resistance to chrysanthemum white rust (CWR), a pathogen of quarantine status in the whole world caused by Puccinia horiana Henn., visualization and microscopic examination were used to define six classes identification standards, and the methods of inoculation in vitro were optimized. The results indicated that two methods of inoculation in vitro were both as efficient as that in vivo. Bottled cuttings in vitro were better than petri dished leaves and a best inoculation method in vitro in our study. The optimized method described here is a safe and simple inoculation system to evaluate the susceptibility of chrysanthemums to CWR.