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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 167-171.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

抑制消减杂交法研究复等位基因遗传的

冀瑞琴 宋倩 辛喜凤 周雪 冯辉   

  • 收稿日期:2011-03-14 修回日期:2011-03-24 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    国家自然科学基金;中国博士后科学基金;辽宁省教育厅实验室专项

The Molecular-mechanism Research of Multiple-Allele-Gene Controlled Male-Sterile in Chinese Cabbage (Brassica rapa L. ssp. chinensis) by Using Suppression Subtractive Hybridization

  • Received:2011-03-14 Revised:2011-03-24 Online:2011-09-05 Published:2011-09-05

PDF (PC)

1006

摘要：

AB01是本课题组培育的复等位基因遗传的核雄性不育大白菜甲型“两用系”，目前已建立了一套该材料的应用技术体系，但其不育分子机制尚不明确。本研究以AB01的不育株和可育株为材料，利用抑制差减杂交技术构建了正反抑制差减cDNA文库，并通过测序及生物信息学手段寻找育性相关基因，以此来推断该材料的不育分子机制。研究中共找到27个差异表达基因，其中25个基因在NCBI数据库中均有同源序列，这些基因中7个与花发育相关，5个与脂类代谢相关，3个与活性氧及能量代谢相关，3个与光合作用及叶绿体合成相关，其余7个为功能未知基因。由此推测复等位基因遗传的核雄性不育大白菜不育的发生与脂类、能量代谢及光合作用有关。

关键词: 绿豆品种, 绿豆品种, 芽用特性, 性状, 地区差异

Abstract:

AB01, the two-type line of multiple-allele inherited male-sterile Chinese cabbage (Brassica rapa L. ssp. chinensis ) was bred by our research group, the technology system of this material had been build, but its sterile molecular mechanism was not clear yet. In this research, sterility and fertility cDNA libraries including 27 differentially expressed clones were constructed using the fertile and sterile buds of AB01 by the suppression subtractive hybridization (SSH). According to BLAST screening and functional annotation, 25 ESTs were homology to known sequence of the databases at the National Center for Biotechnical Information (NCBI). The 25 genes, with homology to known proteins, could be divided into 4 groups (7 flowers developing-related genes, 5 lipid metabolism, 3 energy metabolism genes, 3 chloroplast related genes and 7 encode the unclassified proteins). The results suggested the molecular mechanism of multiple-allele-gene controlled male-sterile Chinese cabbage was related with lipid metabolism, energy metabolism and photosynthesis progress.