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中国农学通报 ›› 2011, Vol. 27 ›› Issue (21): 68-72.

所属专题: 油料作物

• 农学 农业基础科学 • 上一篇    下一篇

磷素对不同大豆品种籽粒异黄酮含量的影响

张勇 接伟光 蔡柏岩 白莉 王丽阳 李二平   

  • 收稿日期:2011-03-21 修回日期:2011-06-14 出版日期:2011-08-25 发布日期:2011-08-25

Effect of Phosphorus on the Content of Kernal Isoflavone in Different Soybean Varieties

  • Received:2011-03-21 Revised:2011-06-14 Online:2011-08-25 Published:2011-08-25

摘要:

为探索磷素对不同大豆品种籽粒异黄酮含量的影响,寻找不同基因型大豆品种最佳施磷水平,以期提高大豆籽粒异黄酮的含量,改善其品质。选用‘黑农48’(高蛋白品种)、‘黑农37’(中间型品种)、‘黑农44’(高油品种)3个大豆品种作为试验材料。采用盆栽,在每kg土壤施N和K2O各为0.033 g基础上,设P1、P2、P3、P4 4个P处理(即每kg土壤分别施P2O5 0、0.033、0.067、0.100 g)。采用紫外分光光度法测定不同大豆品种籽粒总异黄酮的含量。结果表明:同一品种P2处理大豆总异黄酮含量显著高于P1、P3、P4处理,‘黑农37’、‘黑农44’、‘黑农48’3个品种大豆P2处理大豆总异黄酮含量分别比对照组增加3.7%、4.3%、3.8%;不同品种同一处理都是‘黑农44’总异黄酮含量最高;在12个处理组合中‘黑农44’P2处理总异黄酮含量最高,3个大豆品种异黄酮含量在品种间和施磷处理间差异显著。施磷对3个大豆品种异黄酮含量有影响,适宜的施磷量有利于提高大豆籽粒异黄酮的含量。

关键词: 可溶性蛋白, 可溶性蛋白

Abstract:

In this study, the effect of phosphorus on the content of kernal isoflavone in different soybean varieties was analyzed. The best phosphorus levels of different genotype soybean varieties were explored, in order to increase the content of kernal isoflavone and improve their qualities. Three soybean varieties (‘Heinong-48’, high-protein cultivar; ‘Heinong-37’, middle cultivar; and ‘Heinong-44’, high-oil cultivar) were investigated. The soybean seeds were planted in pots and fertilized with 0.033 g of nitrogen (N) and potassium oxide (K2O) per kg soil. Four levels of phosphorus (P) treatment were designed, which were P1, P2, P3 and P4 (i.e., 0, 0.033, 0.067, and 0.100 g of phosphorus pentoxide (P2O5) per kilogram of soil). The content of total kernal isoflavone in different soybean varieties was determined by ultraviolet spectrophotometry. The results showed that the content of total isoflavone under the P2 treatment was significantly higher than that of P1, P3 and P4 treatment in the same variety of soybean. The content of total isoflavone under the P2 treatment in the three soybean varieties (‘Heinong-37’; ‘Heinong-44’; ‘Heinong-48’) compared to the control group was increased by 3.7%, 4.3%, 3.8%, respectively. It showed that the content of total isoflavone in ‘Heinong-44’ was the highest under the same treatment in different soybean varieties. In the 12 treatment combinations, the content of total isoflavone in ‘Heinong-44’ was the highest under the P2 treatment. Significant differences were observed in the content of isoflavone between the varieties and levels of P treatments. These findings suggest that phosphorus affects the total content of isoflavone in the three soybean varieties, and appropriate amounts of P fertilization might contribute to improving the content of kernal isoflavone.

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