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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (29): 72-75.

所属专题： 生物技术； 水产渔业

• 水产 渔业 • 上一篇    下一篇

对虾白斑综合症病毒（WSSV）囊膜表面蛋白的研究

秦崇涛 胡向明 杨丰 潘大仁   

  • 收稿日期:2011-05-24 修回日期:2011-07-18 出版日期:2011-11-15 发布日期:2011-11-15
  • 基金资助:

    国家自然科学基金

Study on the Envelop Surface Protein of White Spot Syndrome Virus（WSSV）

qin chongtao   

  • Received:2011-05-24 Revised:2011-07-18 Online:2011-11-15 Published:2011-11-15

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摘要：

利用生物素标记转移技术对牛血清白蛋白（BSA）和凡纳滨对虾的鳃细胞膜蛋白进行标记，研究其与对虾白斑综合症病毒（WSSV）之间的相互作用。发现分子量在50-56 KD之间的两种蛋白质位于WSSV的表面，并且与鳃细胞膜蛋白具有特异性的相互作用。根据分子量推测可能是病毒的两种膜蛋白，VP52A和VP56。

关键词: 复合模式, 复合模式, 大扁杏, 光合作用, 水分利用效率

Abstract:

In this study, using the biotin label transfer technique, we found that the biotin label was transferred from BSA or gill member protein of Litopenaeus vannamei to the envelop protein of WSSV. Western blotting showed that two proteins whose molecular weight among 50-55KD were labeled with biotin. It was concluded that the two proteins was location on the surface of the WSSV viron and could interact with the gill member protein of Litopenaeus vannamei. They were estimated to be VP52A and VP56.

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