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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 282-287.

• 植物保护 农药 • 上一篇    下一篇

灰黄霉素对尖孢镰刀菌抑制作用的研究

刘芸 朱育菁 陈清西 李智聪 胡桂萍 于晓杰 刘波   

  • 收稿日期:2011-06-21 修回日期:2011-08-30 出版日期:2011-10-25 发布日期:2011-10-25
  • 基金资助:

    灰黄霉素的藕合修饰及其对植物重要真菌病害

Antifungal activity of griseofulvin against Fusarium oxysporum, pathogen of crop Fusarium wilt disease

  • Received:2011-06-21 Revised:2011-08-30 Online:2011-10-25 Published:2011-10-25

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摘要：

为进一步扩展灰黄霉素的农业应用范畴，选择采自甜瓜、黄瓜、西瓜、苦瓜、辣椒和西红柿的不同专化型的10株致病性尖孢镰刀菌作为试验对象，系统研究了灰黄霉素对尖孢镰刀菌的抑制作用。分别采用抑菌圈法和抑菌率法，测定灰黄霉素对不同寄主来源的尖孢镰刀菌的抑制作用，并显微观察灰黄霉素对尖孢镰刀菌菌丝生长的影响。结果表明灰黄霉素对不同寄主来源的尖孢镰刀菌具有良好的广谱抑制作用，但对不同菌株抑制效果差异显著，其抑制中浓度IC50为0.42~2.81 mM。尖孢镰刀菌经4 mM灰黄霉素处理后，菌丝变得稀疏、畸形、膨大、扭曲，从而影响其生长。因此，灰黄霉素可用于开发防治作物枯萎病的新型生物农药。

关键词: 环太湖河流, 环太湖河流, 入出湖口, 沉积物, 污染物

Abstract:

Abstract: 【OBJECTIVE】The antifungal activities of greseofulvin The antifungal activities of greseofulvin against ten isolates of Fusarium oxysporum, pathogen of crop Fusarium wilt disease were investigated with an aim to extend the application of griseofulvin for controlling the disease. The antifungal activities were determined in a set of inhibition ratio and inhibition ring tests with the ten isolations of F. oxysporum as indicators, which were isolated from oriental melon, cucumber, water melon, pepper and tomato. The effect of griseofulvin on the growth of the isolations was observed under microscopes. The results revealed that the ten isolations presented significantly different but high sensibility to griseofulvin. The half-inhibitory concentration (IC50) values of greseofulvin against the fungi were calculated as 0.42-2.81 mM. Treated with 4 mM griseofulvin, F. oxysporum was found to be induced degenerative changes of hyphal morphology. The study indicated that griseofulvin may be a new promising biological agent or a lead compound for novel antifungal agent for controlling Fusarium wilt disease.

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