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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (7): 171-175.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

香蕉枯萎病病原菌海藻糖合成酶基因tps1

杨腊英 黄小娟 谢玉萍 黄俊生   

  • 收稿日期:2011-10-11 修回日期:2011-11-07 出版日期:2012-03-05 发布日期:2012-03-05
  • 基金资助:

    公益性行业（农业）科技专项经费项目;中央级公益性科研院所基本科研业务费专项

Cloning and Induced Expression Analysis of tps1 Gene of Fusarium Oxysporum f. sp.

  • Received:2011-10-11 Revised:2011-11-07 Online:2012-03-05 Published:2012-03-05

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摘要：

为了解tps1基因在尖镰刀菌古巴专化型生理小种1号和生理小种4号之间及与其他镰刀菌之间的差异，分析推测tps1基因与两生理小种之间的寄主选择性差异的关系，通过比对现有镰刀菌属的海藻糖合成酶基因序列，参照尖孢镰刀菌番茄专化型的tps1基因序列设计并合成引物，采用PCR和RT-PCR方法扩增了两个生理小种的tps1基因并测序进行比较分析，同时对香蕉与粉蕉苗诱导两个生理小种后的tps1基因表达量变化进行分析。结果表明，两个生理小种tps1基因全长均为1660 bp，开放阅读框均为1605 bp，编码535个氨基酸，基因序列间仅存在两个碱基位点的差异，编码蛋白间无差异；两生理小种的tps1基因序列与镰刀菌属其他种间存在较大的差异，经寄主诱导后的两个生理小种的tps1基因表达量呈现不同的变化。从tps1基因序列及其编码蛋白的氨基酸序列在两生理小种之间的差异性分析结果推测，两个生理小种寄主选择性差异与tps1基因并无明显对应关系，这为进一步研究tps1基因与病菌在无寄主下的长期生存的关系奠定了基础。

关键词: 预测, 预测

Abstract:

In order to realize the difference of tps1 gene between Fusarium oxysporum f. sp. FOC race 1 and race 4 and other Fusarium oxysporum f. sp. and analyze whether the host selective difference between FOC race 1 and race 4 were related with tps1 gene. The tps1 genes of race 1 and 4 were amplified by Polymerase Chain Reaction and Reverse Transcription Polymerase Chain Reaction and sequence, the gene sequences and the encoded protein sequences were compared by DNA Star software, and also analyzed the expression quantity alteration of tps1 after induction with Musa AAA Cavendish or Musa ABB Pisangawak. The full length of tps1 from FOC race 1 and race 4 were both 1660 bp, with their open reading frames 1605 bp, encoding 535 amino acids. There were only two base differences among the sequences of the tps1 gene of FOC race 1 and race 4 and their amino acid sequences were the same. The tps1 gene sequences of these two races were different from those of other specialized forms or species of Fusarium oxysporum f. sp. It was inferred from the differences of the tps1 gene sequence and protein sequences from FOC1 and FOC4 isolates that there was no obvious relation between tps1 gene and the host selective preference of FOC1 and FOC4. These results would help to know the relation of tps1 genes and FOC long-term survival without host in the future research.