欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 230-235.doi: 10.11924/j.issn.1000-6850.2011-2980

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

葡萄炭疽病菌SRAP遗传多样性分析

李利霞 赵奎华 刘长远 梁春浩 关天舒 王辉 杨卫娟 马晓飞   

  • 收稿日期:2011-10-14 修回日期:2011-12-12 出版日期:2012-04-25 发布日期:2012-04-25
  • 基金资助:

    国家农业科技成果转化资金项目

Analysis on Genetic Diversity of Colletotrichum gloeosporioides Based on SRAP

  • Received:2011-10-14 Revised:2011-12-12 Online:2012-04-25 Published:2012-04-25

PDF (PC)

827

摘要：

探讨葡萄炭疽病菌的变异和群体结构，为进一步深入研究葡萄炭疽病的发生、流行及防控技术提供理论依据。采用SRAP分子标记技术对不同地区的25个葡萄炭疽菌(Colletotrichum gloeosporioides)菌株进行遗传多样性分析。利用4个菌株从100对引物组合中筛选出6对扩增条带多样性丰富、稳定性较好的引物组合；对供试的25个菌株进行SRAP扩增，共得到164条清晰可辨的条带，其中多态性条带为156条，多态性比率为95.12%；利用NTSYS-2.1软件进行病原菌的聚类分析，其相似性系数在0.61~0.95之间。不同地区葡萄炭疽菌的亲缘关系较近，遗传多样性丰富，但各菌株间存在遗传差异，且菌株之间的差异与地理来源无明显相关性。

关键词: 水貂, 水貂, 哺乳症, 脂肪酸, 胰岛素抵抗

Abstract:

In order to provide a theoretical basis for the occurrence, epidemic and control technologies of the disease, the variation and genetic structure of Colletotrichum gloeosporioides were explored. Sequence related amplified polymorphism (SRAP) analysis was used to detect the genetic variation in populations of the 25 C. gloeosporioides in different areas. Among 100 combinations of primer pairs screened, 6 primer pairs that amplified consistent polymorphic bands with DNA of 4 isolates were generated. They were further applied to amplify the genomic DNA of the 25 tested isolates. A total of 164 discernible bands were obtained, 156 of which showed polymorphism, and similarity coefficient was 95.12%. The dendrogram was constructed with the software NTSYSpc Version2.1 and the similarity coefficients were ranged from 0.61-0.95. The result indicated there was a close genetic relationship and abundant genetic diversity. Nonetheless, rich genetic variation existed among the tested isolates and there was no correlation between SRAP group and the geographic origin.