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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (24): 179-182.

所属专题： 生物技术； 玉米

• 生物技术科学 • 上一篇    下一篇

玉米淀粉分支酶SBEⅠ基因RNAi干扰载体的构建

张颖君 高慧敏 刘茜 胡梦芸 李辉   

  • 收稿日期:2011-10-17 修回日期:2012-02-14 出版日期:2012-08-25 发布日期:2012-08-25
  • 基金资助:

    河北省农林科学院前期预备项目

Construction of RNAi Vector of Starch Branching Enzyme SBEⅠ of Maize

  • Received:2011-10-17 Revised:2012-02-14 Online:2012-08-25 Published:2012-08-25

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摘要：

淀粉分支酶(SBE)催化葡萄糖以α-(1,6)糖苷键连接，形成分支结构。SBE有3种同工酶形式：SBEⅠ、SBEⅡa和SBEⅡb，目前的研究主要集中在SBEⅡb上，而对于SBEⅠ在淀粉合成的作用报道极少。为了明确SBEⅠ在玉米淀粉合成中的作用，扩增了玉米SBEⅠ基因的337 bp片段，以pMD19-T为中间载体，pCambia3301为目标载体，构建玉米SBEⅠ基因的RNAi载体，命名为pC3301-SBEⅠ。通过限制性内切酶酶切鉴定，表明pC3301-SBEⅠ载体构建正确，可用于农杆菌介导的玉米自交系转化工作。

关键词: 草本植物, 草本植物, 群落特征, 植物多样性, 乌兰布和沙漠

Abstract:

The strach branching enzyme (SBE) can introduce α-1,6-glucan linkages to α-1,4-glucan, which make the starch brached. There are three isoforms of SBE: SBEⅠ, SBEⅡa and SBEⅡb. Many studies were focused on SBEⅡb, and few studies on SBEI. In order to clarify the role of SBEI in starch synthesis, the 337 bp fragment of SBEⅠ gene was amplified based on the sequence AF07272, and used the pMD19-T vector as interim vector the RNAi hairpin of SBEⅠ gene was constructed into pCambia3301 vector, and the new vector was named as pC3301-SBEⅠ. Through the identification of restriction endonuclease, the pC3301-SBEⅠ was proved to be correct. The new vector was used to transfer maize mediated by agrobacterium tumefaciens.

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