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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (21): 242-247.doi: 10.11924/j.issn.1000-6850.2011-3565

所属专题： 植物保护

• 植物保护 农药 • 上一篇    下一篇

基质固相分散萃取-气相色谱法同时检测丹皮中有机氯和拟除虫菊酯农药残留的研究

高倩 花日茂 王兴琴 马鑫   

  • 收稿日期:2011-11-29 修回日期:2012-02-03 出版日期:2012-07-25 发布日期:2012-07-25
  • 基金资助:

    MSPD-GC-ECD法检测丹皮中有机氯等农药残留

Simultaneous Determination of Organochlorines and Pyrethroid Pesticide Residues in Cotex mouta Based on Matrix Solid Phase Dispersion and Gas Chromatography

  • Received:2011-11-29 Revised:2012-02-03 Online:2012-07-25 Published:2012-07-25

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摘要：

采用基质固相分散萃取-气相色谱(MSPD-GC)技术，建立丹皮中有机氯和拟除虫菊酯农药多残留分析新方法。以回收率为指标，采用正交设计优化选择填料种类，样品与填料量的比例、淋洗液的极性。丹皮样品与中性氧化铝(m/m，1:2)充分研磨5 min后装入10 mL玻璃层析柱内，通过10 mL乙酸乙酯洗脱，11种农药在0.02、0.1、1 mg/kg 3个添加水平的平均回收率为77.69%~110.7%，相对标准偏差均低于11.6%，方法检出限在0.1~3.4 μg/kg范围。该方法可同时监测丹皮中有机氯、拟除虫菊酯类等农药的残留水平，方法简便。

关键词: PCR, PCR

Abstract:

A new method was proposed to measure organochlorines and pyrethroid pesticide residues in Cotex mouta simultaneously by gas chromatography and matrix solid-phase dispersion. The recovery was used to evaluate the extraction and clean-up method, extraction conditions were optimized using orthogonal experimental design, including stuff, solvent and their dosage. Dry ground Cotex mouta samples and neutral alulllinum oXide (1:2) were homogenized in a mortar for 5 min and then packed into a 10 mL of glass column by eluting with ethyl acetate solution. The qualitative and the quantitative analysis of samples was accomplished using gas chromatography with ECD (electronic capture detector). Mean recoveries for 11 different pesticides in Cotex mouta were found in the range of 77.69%-110.7% at the spiked levels of 0.02, 0.1 and 1 mg/kg, and relative standard deviation (RSD) were all lower than 11.6%. The limits of detection (LOD) of the method ranged between 0.1-3.4 μg/kg. The proposed method could detect organochlorines and pyrethroid pesticides in Cotex mouta.