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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (26): 71-75.

所属专题： 生物技术

• 水产 渔业 • 上一篇    下一篇

黑龙江草蜥消化道5种内分泌细胞的免疫组织化学定位研究

周立丽 彭一良 李淑兰   

  • 收稿日期:2012-03-21 修回日期:2012-05-09 出版日期:2012-09-15 发布日期:2012-09-15
  • 基金资助:

    哈尔滨师范大学科技发展预研项目

Immunohistochemical Localization of 5 Kinds of Endocrine Cells in Digestive Tract of Takydromus amurensis

  • Received:2012-03-21 Revised:2012-05-09 Online:2012-09-15 Published:2012-09-15

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摘要：

为了研究黑龙江草蜥(Takydromus amurensis)消化道内生长抑素(SS)、胃泌素(Gas)、胰高血糖素(Glu)、胰多肽(PP)和P-物质(SP)5种内分泌细胞免疫组织化学的定位。采用免疫组织化学ABC法。结果表明：在黑龙江草蜥消化道中，SS细胞除食管部外，其余各部位均有所分布，其分布密度胃部高于肠部。GAS细胞仅见于胃幽门部、十二指肠和空肠。Glu细胞分布于胃体、幽门和小肠，且幽门密度明显高于其他2处，PP细胞在胃体、幽门和十二指肠有分布，其中以十二指肠处的分布密度最高，SP细胞只在胃部检测到。内分泌细胞形态多样，多呈圆形、锥体形、梭形。黑龙江草蜥消化道内分泌细胞的密度分布与其消化道各部位的功能有关，它们的形态与其内、外分泌功能是相适应的。

关键词: 抗旱性, 抗旱性

Abstract:

To study the immunohistochemical localization of 5 kinds of endocrine cells including somatostatin (SS), gastrin (Gas), Glucagon (Glu), pancreatic polypeptide (PP) and substance P-(SP) in the digestive tract of Takydromus amurensis. The ABC immunostaining technique was used. The results showed that: SS positive cells distributed throughout the digestive tract except the oesophagus. The stomach’s distribution of density was higher than that in the intestine. Gas cells were observed only in the pylorus, duodenum and jejunum in the digestive tract of Takydromus amurensis. Glu cells were observed in the stomach, pylorus and small intestine, and the distribution of the density of Glu cells in the pylorus was obviously higher than the two other parts. PP cells were detected in the stomach, pylorus and duodenum in the digestive tract of Takydromus amurensis, and the distribution density was the highest in the section of duodenum. SP cells were found only in the stomach. The forms of endocrine cells were various, mostly round, cone-shaped, spindle-shaped. The distributive density of endocrine cells was related to its function of each part of the digestive tract. The morphology of which were conformable with the endocrine and exocrine functions.

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