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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (8): 184-188.doi: 10.11924/j.issn.1000-6850.2012-1667

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

一组混合菌群还原硫酸盐的特性

马忠友 邓盾 汪建飞 谢越   

  • 收稿日期:2012-05-02 修回日期:2012-06-13 出版日期:2013-03-15 发布日期:2013-03-15
  • 基金资助:
    国家自然科学基金“双孢蘑菇镉耐受基因突变体库的构建和基因克隆”(31100070)；安徽科技学院稳定人才基金“纤溶酶高产菌株的分离、鉴定”(ZRC2011299)；安徽科技学院生物学重点建设学科(AKXK20102-1)；安徽省农业科技成果转化基金项目“规模化养殖场废弃物生产有机肥关键技术及产业化开发”(10140306017)。

Study of the Physiology on Sulfate Reducing Bacteria

  • Received:2012-05-02 Revised:2012-06-13 Online:2013-03-15 Published:2013-03-15

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摘要： 为了利用硫酸盐还原法消除硫酸盐和重金属离子对环境的污染，从凤阳城区排水沟中富集和驯化获得硫酸盐还原菌菌群，研究了硫酸盐还原菌群对含硫氧酸盐的还原，以及温度、pH、盐度、氧气和Fe2+等因素对菌群还原硫酸盐能力的影响。结果表明，该菌群可以利用乳酸钠还原硫代硫酸盐、硫酸盐，不能还原亚硫酸盐；最适合的生长温度为36℃，pH 7.5，可在3%的氯化钠浓度下生长；在兼性厌氧条件下对硫酸盐还原能力最强；硫酸盐还原率随着Fe2+浓度的增加而增大。菌群接种量为10%，FeSO4初始浓度为2000 mg/L，培养30 h后硫酸盐还原率达到95%以上。

关键词: 抗性, 抗性

Abstract: In order to utilize sulfate-reducing method on the treatment of heavy metal wastewater and organic wastewater containing sulfate, sulfate reducing bacteria (SRB) were enriched from the long-term polluted sewer in Fengyang, and their removing sulfate abilities were studied in the different conditions, such as: sulfur source, temperature, pH value, salinity, oxygen and the concentration of Fe2+ . The results showed that, CH3CHOHCOONa could be used as a carbon source to reduce sulfate and thiosulfate, and sulfite could not be reduced. The optimum conditions for SRB growth were found to be at 36℃, pH 7.5. SRB were able to grow in the NaCl concentration of 3%, meanwhile, their removing sulfate abilities was the best in facultative anaerobic; the rate of sulfate reducing was increasing with the increased Fe2+ concentration. The rate of sulfate reducing would reach above 95% after thirty hours when SRB were inoculated by 10% and the concentration of FeSO4 was 2000 mg/L.